2. The promoter (P) is the start site of transcription through the binding of RNA polymerase before actual mRNA production. Mutated promoters (P) cannot bind RNA polymerase, so no mRNA is made. In the table below, make predictions about the expression of ß-galactosidase and permease for different partial diploid (F') genotypes. Do this to mark where there is no E. coli growth on two different types of media - one without lactose, and one with. Use a - enzyme produced, and a + to mark where the enzyme is made. The first genotype has all wild-type alleles, and has b- filled in as an example. Genotype I+P+O+Z+Y+ I+P+OcZ+Y- I-P*O*Z*Y*+ I+P+O+Z-Y/IPO*Z*Y* IP OCZ+Y/IPO+Z-Y* ISP+O+Z+Y/I+P+O+*Z-Y* Lactose absent B-galactosidase + Permease + Lactose present B-galactosidase Permease

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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1. Draw a diagram of the lac operon.
PO
I+P+O+Z+Y+
I+P+OcZ+Y-
I-P+O+Z+Y+
Lab section (circle):
lacz gene lac Y gene lacA gene
I+P+O+ZY/IPO+Z+Y-
2. The promoter (P) is the start site of transcription through the binding of RNA polymerase before actual mRNA
production. Mutated promoters (P) cannot bind RNA polymerase, so no mRNA is made. In the table below, make
predictions about the expression of ß-galactosidase and permease for different partial diploid (F') genotypes. Do this for
E. coli growth on two different types of media - one without lactose, and one with. Use a to mark where there is no
enzyme produced, and a + to mark where the enzyme is made. The first genotype has all wild-type alleles, and has been
filled in as an example.
Genotype
IPOCZ+Y/I-P+O+Z-Y+
ISP+O+Z+Y/I+P+O+Z*Y*
CR 1:00
Lactose absent
B-galactosidase
Permease
R 3:-00
+
R 6:30
Lactose present
B-galactosidase
F 10:00
Permease
Transcribed Image Text:1. Draw a diagram of the lac operon. PO I+P+O+Z+Y+ I+P+OcZ+Y- I-P+O+Z+Y+ Lab section (circle): lacz gene lac Y gene lacA gene I+P+O+ZY/IPO+Z+Y- 2. The promoter (P) is the start site of transcription through the binding of RNA polymerase before actual mRNA production. Mutated promoters (P) cannot bind RNA polymerase, so no mRNA is made. In the table below, make predictions about the expression of ß-galactosidase and permease for different partial diploid (F') genotypes. Do this for E. coli growth on two different types of media - one without lactose, and one with. Use a to mark where there is no enzyme produced, and a + to mark where the enzyme is made. The first genotype has all wild-type alleles, and has been filled in as an example. Genotype IPOCZ+Y/I-P+O+Z-Y+ ISP+O+Z+Y/I+P+O+Z*Y* CR 1:00 Lactose absent B-galactosidase Permease R 3:-00 + R 6:30 Lactose present B-galactosidase F 10:00 Permease
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