16. During early life many infants receive milk from the mother. One of the major components ofmilk is lactose.a) Consider the Lac operon in E. coli, and state what the expression of Lac Y would be in I) E.coli WT (wild type, everything is functional) and II) E. coli whose lacl gene has been delet-ed, when each of the 2 strains are growing in culture flasks that contain high glucose andhigh lactose. Hint: choose among 'high expression; low expression; no expression'for I) andII).1)II)b) In the graph below, draw the expression of LacY over time in strains I and II, when each of the2 strains are growing in culture flasks that contain high glucose and high lactose, as shown inpart a). Hint: you can draw the curves on a piece of paper and copy and paste a picture of it,if it is difficult to draw the graph directly here. If you do so and you have a Mac, make sure toconvert your exam into PDF by doing "Print > Save as PDF"; do not “Export as PDF".time (min)Make sure to label which curve refers to strain I and which one refers to strain II.LacY expression

the lacI gene is a regulatory gene for lac operon It produces a protein that blocks RNA polymerase…

Answered: coli WT (wild type, everything is…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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The pathway for arginine biosynthesis in Neurospora crassa involves several enzymes that produce a series of intermediates as shown. O O O O ornithine citrulline ARG-E arginosuccinate arginine N-acetylornithine arginine You did a cross between ARG-E ARG-H* and ARG-E* ARG-H¯¯ Neurospora strains and identified an Arg- strain from an NPD tetrad. (Assume that Neurospora forms tetrads in the same way yeast do.) Which compound would rescue growth of this Arg- spore? N-aceltylornithine ARG-F ornithine citrulline ARG-G ARG-H → argininosuccinate
Beer's Law to determine Protein Concentration You have purified a recombinant form of the p53 protein from E. coli and determined the A280 to be1.35. Calculate the molar and mass concentration of the purified protein if the extinction coefficientand molecular weight of p53 is 35,410 M-1 cm-1 and 43,653 Da, respectively (l = 1 cm).
E. coli cells which are mutant for the y gene cannot accumulate ? in large enough amounts to use it as an energy source. galactose lactose beta-galactosidase CO2 O beta-galactoside permease
Please help with this question with a better explanation
In a-c below, will the residue on the right-hand side increase or decrease the pKa of the residue on the left-hand side? Explain.
Wild type genomic sequence of a portion of a gene and the wild type sequence of portion of the protein gene product. The protein sequence only matches partially. ТАT AAA GGG СGA ССА ССТ GсT AАT GGG АСт тTG AGG Tyr Lys Gly Arg Pro Pro Ala Pro Arg Gln Tyr Trp Note: The five amino acids at the amino end of the WT protein match the coding sequence perfectly, but not the six at the carboxyl end. This is for an obvious reason that you should figure out before starting the problem. One of the difficulties with this problem is that you do not have the DNA sequence for the carboxyl end of the protein, but that should not be a problem! And, so far, there are no mutations; nothing unusual, it is just a normal eukaryotic gene!! A mutant is found that has the following protein sequence in that part of the protein: Tyr Lys Gly Arg Thr Cys Ser Premature Stop. What is the likely mutation? between A12 and C13 insert T. between C13 and C14 insert T. A15 -> del. C13 and C14 -> del. G8 -> T. G20 -> A. T21…
When considering the mutational options for a nucleotide such as thymine, there is a single transition and two transversion type mutations that are possible, which is why we observe transversions to occur more frequently in nature when genomes are examined. O True O False
help
. You need to prepare 500 μL of a 250 µg/mL bovine gamma globulin solution, for one of your protein standards in the Bradford assay. The available bovine gamma globulin in the lab is in the form of a 2 mg/mL stock solution, and it can be diluted with deionized water. Describe or show below how you would create your desired 250 µg/mL solution.
Fig 2 shows gastric organoids cultured from a TffCre; XYZI/fl mouse. Ki67 has been used to identify proliferating cells (green). Tamoxifen indicates exposure to tamoxifen to induce recombination of the TffCre. No Tamoxifen Ki-67 3. What is the consequence of deleting XYZ in gastric organoids, and does this suggest it is an oncogene or a tumour suppressor? Explain your reasoning. DX Equation Editor 4. IWP inhibits the secretion of Wnt ligands from the organoids. Therefore, do Wnt ligands promote or inhibit proliferation of XYZ deficient organoids? Explain your reasoning. X Equation Editor Tamoxifen Q Tamoxifen + IWP A- A I B IU S x, x² Styles Font Size Font Words: 0/250 张 A- A-T BIUS X, x² Styles ▾ Size
Name on methoud that could have been used to deterine where the DMR are in the figures. And How is expression of gene in the IMR90 strain (picture in green) effected at differentially methylated regions pictured in the figure?

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