You are working with a laboratory strain of E.coli that contains a 10 base-pair deletion in the Lac operator region. You grow this mutated strain of E.coli in a variety of media conditions as shown below for two hours. After two hours you are able to measure the amount of beta- galactosidase activity (from the lacZ gene product) in each cell culture. Media condition C High lactose, high glucose 100 units 100 units 100,000 units 100 units High lactose, no glucose 100 units 100,000 units 100 units 100,000 units No lactose, no glucose 100 units 1001000 units 100 units 100 units No lactose, high glucose 100 units 100 units 100,000 units 100 units Which column best represents the data you would expect with a wild-type or normal strain? C.

Q: Explain why large amounts of transcript result when a bacterium with a lac Operon is in a medium…

A: Transcription is the process by which RNA is synthesized from DNA. The messenger RNA or mRNA…

Q: From the result shown, what might be the histidine biosynthetic pathway (the order of enzymes)?

A: Histidine biosynthesis is an ancient metabolic partway present in bacteria, archaea, lower…

Q: Here is a list of knockout gene mutations that are desired to make in E. coli genome. You should…

A: A number of knockout gene mutations in the E. coli genome are shown to us in this scenario. Each…

Q: Consider a mutant of E. coli that has an inactivating mutation in the gene for adenylate cyclase…

A: Inactivating mutations, also known as loss-of-function mutations, cause the gene product to have…

Q: Parts A-C show the genotypes for partial diploid strains of the lac operon. Gene names have been…

A: (a) I-P+O+Z+Y:- The I- allele means the lac repressor is inactive, so the lac operon is…

Q: a. Assume that replication has been initiated at that ORI. Provide the sequence of the primer that…

A: DNA is the genetic material of almost all living organisms. It contains information that is…

Q: Compatible ends generated by restriction enzyme can be connected by ligase. Determine which of the…

A: Restriction enzymes are proteins that cut DNA at specific sequences. These sequences are usually…

Q: Based on the following wild type DNA sequence, indicate if each of the mutations should be…

A: A mutation is a change in a DNA sequence. Mutations can result from DNA copying mistakes made during…

Q: A site-directed mutagenesis experiment was done on the catalytic triad of the serine protease…

A: Mutagenesis is that the method through that mutations in DNA molecules arise. Mutations in DNA will…

Q: An E. coli lac- mutant (a mutant that cannot grow in the medium containing lactose as only carbon…

A: Operon, is a bacterial and viral genetic regulation system in which genes coding for functionally…

Q: You want to find E. coli genes necessary for lactate metabolism. You have a population of…

A: Here, we have to find mutations in genes necessary for lactate metabolism. So, first we will have to…

Q: E. coli K12 genomic DNA (expected PCR product was 3075bp). For expression the gene was cloned into…

A: The information you provided suggests that a gene was cloned into the expression plasmid pET28a, and…

Q: The lactose operon in E. coli comprises two structural genes and three regulatory components.…

A: β-galactosidase activity Permease activity Genotypes Glycerol Lactose Glycerol Lactose A - + -…

Q: Explain how the lac operon can be used to express a recombinant protein in an E. coli cell.

A: Since you have asked multiple question, we will solve the first question for you. If you want any…

Q: Why is the ErbB family pathway an important pathway for researchers that target cancer therapy.…

A: Numerous human cancers are thought to be caused by aberrant activation of the ErbB family of…

Q: Suppose that E. coli sustains a mutation in its gene for the lac operon repressor making the…

A: When lactose is not present in the medium operon does not transcribe because of the presence of lac…

Q: 4). Mutants unable to synthesize an essential compound G were tested with related compounds D, E,…

A: It is used to test mutants that are not able to produce an essential compound and to examine their…

Q: Calculate the concentration of lac repressor, assuming that one molecule is present per cell. Assume…

A: A set of genes present in E.coli. that regulates the uptake of lactose from the surrounding…

Q: Trp operon of E. coli is an inducible sytem since it turns on in the presence of tryptophan. In most…

A: DNA codes for proteins, which occur in three stages: replication, transcription, and translation.…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.