Initial velocity plots for wildtype PRPP synthetase in the presence of the indicated amounts of [ADP] are shown below. Draw on thisgraph the plot you would expect for the mutated form of PRPP synthetase from the patient with gout in the absence of ADP. Draw asecond curve on the graph the plot you expect to find in the presence of your analog assuming your design is a successful one.[ADP] =150-VΟ μΜ25 μΜ50 ΜΜ100-100 μ.Μ200 μΜ400 μ. Μ50-0+0.1Pi (mM)10100

📌 Detailed Explanations: In the task at hand, we are analyzing an enzyme reaction graph for PRPP…

Answered: Initial velocity plots for wildtype…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Identify the open reading frame for the following sequence: CACAGCCTACTAATGGTGTTGGCTAT Note: When I first attempted this question I identified the open reading frame as starting from ATG. However I was told that my reading frame was wrong and that the frame should include an asparagine group but how it that possible?
Equation 7a of the text, (Umax/KM1) [S] (v'max/KM2) [P] 1+ [S]/KM1 + [P]/KM2 - gives the expression for the rate of formation of product by a modified version of the Michaelis-Menten mechanism in which the second step is also reversible. Derive the expression and find its limiting behaviour for high and low concentrations of substrate.
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The purified OXA-M290 enzyme can now be tested to determine which β-lactamase inhibitor is most effective. This inhibitor could be prescribed in combination with a β-lactam antibiotic to treat the infection caused by the E. coli KGH1 strain. Before testing inhibitors against OXA-M290, the kinetic activity of this enzyme must first be measured. The activity of OXA-M290 is measured using nitrocefin, a chromogenic β-lactam antibiotic. When nitrocefin is hydrolyzed by a β-lactamase, it changes from yellow to red in colour. The nitrocefin hydrolysis product has an extinction coefficient of 20,500 M-1 cm-1 at 486 nm. The hydrolysis of 60 μM nitrocefin by 1 nM OXA-M290 is monitored using a microplate reader. The absorbance of the wells in the plate is measured at 486 nm every 30 seconds. This experiment is carried out with three replicates, generating the following data: Time (min) Absorbance of Replicate 1 Absorbance of Replicate 2 Absorbance of Replicate 3 0.5 0.0984…
Binding of a transcriptional activator to a promoter according to the Hill equation can be analyzed graphically by plotting the quantity f,([X*]) log10 1- f„([X']), versus log,,[X]. A. Show that this results in a linear relationship between dependent and independent variables and give the slope and intercept in terms of the Hill coefficient n and apparent dissociation constant K. B. Generate such linearized plots corresponding to hypothetical binding isotherms for the cases n = 1, 2, 3, 4 with K = 1:10-9 M.
28. In a transformation experiment, DNA is collected from an E. coli donor strain of genotype cys(+) leu(-) thr(+) and used to transform a recipient of genotype cys(-) leu(+) thr(-). Initially, the treated recipient population is plated on a minimal medium supplemented with threonine. Many colonies are obtained. What are possible genotypes of these colonies? A. cys(-) leu(+) thr(+) B. cys(+) leu(+) thr(-) C. All are cys(+) and either + or - for leu and thr D. All are thr(+) and either + or - for cys and leu E. All are thr(-) cys(-) and either + or - - for CYS
Schizosaccharomyces pombe, also known as "fission yeast," is a powerful model organism in molecular and cell biology. While performing a genetic screen, you discover an auxotrophic S. pombe strain that is unable to synthesize one or more vitamins. The following table represents the key experiments you performed during your genetic screen. Fill in the table with the outcome of each experiment for your mutant strain (using + for growth and - for no growth). Medium Rich media Minimal media Minimal media + all vitamins Minimal media + all amino acids Growth Wild-type + + + + Mutant + + + > > >
When Zidovudine (3'-azidothymidine or AZT) is added directly to an assay measuring the rate of DNA synthesis catalyzed by the AIDS virus reversed transcriptase, AZT is shown to be without significant inhibitory activity. Explain how AZT is metabolized in virus infected cells to convert the nucleoside into a potent and effective reversed transcriptase inhibitor.
Peptide Sequencing Answer numbers 1 and 2. For number 2, show the OVERLAP method.
To identify the critical enzyme resi- (c) ( due:substrate interaction and determinant of sub- D(-2) Arg47 strate specificity, steady-state kinetic studies have D(-4) been carried out by site-specific mutagenesis of the enzyme and by systematically changing amino acid residues in the 11-amino acid phosphotyrosine con- taining substrate that simulate residues 988 – 998 of the epidermal growth factor receptor (EGFR988–998). Asp48 E(-1) A(-3) The table below compares the kinetic parameters of the wild type enzyme with phosphotyrosine pep- tides in which amino acid residues are substituted. L(+1) Тyr46 Gln262 In the adjacent diagram the structural interactions between active site residues are illustrated for the Y(0) first 6 residues of the substrate. Identify which sub- strate residue is most sensitive to substitution and Ser216 justify your conclusion on the basis of the kinetic parameters. Is the decrease in kcat/ KM due to loss of catalytic reactivity or due to suboptimal position- ing…

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