Your are working in a biodiesel production plant and are trying to optimize the production of biodiesel (Fatty Acid Methyl Esters). The enzyme lipase catalyzes a key step in this reaction (See below). You are tasked with mutating a recombinant lipase to improve the efficiency of the reaction to improve yields of the Fatty Acid Methyl Esters. Which kinetic parameters would you calculate to determine the efficiency of the mutate enzymes. Briefly explain the design of an experiment to calculate these parameters. LIPASE + H,C-OH Lipase production Vegetable oil Methanol Biodiesel Transesterification он Lipase -OH OH он Triglyceride Fatty acids Glycerol

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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Your are working in a biodiesel production plant and are trying to optimize the production of biodiesel (Fatty Acid Methyl Esters). The enzyme lipase catalyzes a key step in
this reaction (See below). You are tasked with mutating a recombinant lipase to improve the efficiency of the reaction to improve yields of the Fatty Acid Methyl Esters. Which
kinetic parameters would you calculate to determine the efficiency of the mutate enzymes. Briefly explain the design of an experiment to calculate these parameters.
LIPASE
+ H,C-OH
Lipase production
Vegetable oil
Methanol
Biodiesel
Transesterification
-OH
R
HO.
Lipase
+
R2
HO.
HO,
Triglyceride
Fatty acids
Glycerol
Transcribed Image Text:Your are working in a biodiesel production plant and are trying to optimize the production of biodiesel (Fatty Acid Methyl Esters). The enzyme lipase catalyzes a key step in this reaction (See below). You are tasked with mutating a recombinant lipase to improve the efficiency of the reaction to improve yields of the Fatty Acid Methyl Esters. Which kinetic parameters would you calculate to determine the efficiency of the mutate enzymes. Briefly explain the design of an experiment to calculate these parameters. LIPASE + H,C-OH Lipase production Vegetable oil Methanol Biodiesel Transesterification -OH R HO. Lipase + R2 HO. HO, Triglyceride Fatty acids Glycerol
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