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2C Two homologues of this enzyme from different species both catalyze the reaction SY with distinct Km values. Protein A catalyzes the reaction with Km = 2.0 μM and protein B catalyzes it with Km = 0.5 μM. In an experiment where each enzyme is mixed with 1 μM substrate, the plot of product Y formation over time was obtained. Which curve corresponds to which enzyme?

2C Two homologues of this enzyme from different species both catalyze the reaction SY with distinct Km values. Protein A catalyzes the reaction with Km = 2.0 μM and protein B catalyzes it with Km = 0.5 μM. In an experiment where each enzyme is mixed with 1 μM substrate, the plot of product Y formation over time was obtained. Which curve corresponds to which enzyme?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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2C Two homologues of this enzyme from different species both catalyze the reaction SY with distinct Km values. Protein A catalyzes the reaction with Km = 2.0 μM and protein B catalyzes it with Km = 0.5 µM. In an experiment where each enzyme is mixed with 1 μM substrate, the plot of product Y formation over time was obtained. Which curve corresponds to which enzyme? Time 2D How could we use experiments like the experiment above to determine the Km and Vmax of other homologous enzymes? 2E If a competitive inhibitor with K₁=2 µM were added to a homolog of an enzyme with Km = 2 mM, what would be the resulting Kmapparent? [X]
Transcribed Image Text:2C Two homologues of this enzyme from different species both catalyze the reaction SY with distinct Km values. Protein A catalyzes the reaction with Km = 2.0 μM and protein B catalyzes it with Km = 0.5 µM. In an experiment where each enzyme is mixed with 1 μM substrate, the plot of product Y formation over time was obtained. Which curve corresponds to which enzyme? Time 2D How could we use experiments like the experiment above to determine the Km and Vmax of other homologous enzymes? 2E If a competitive inhibitor with K₁=2 µM were added to a homolog of an enzyme with Km = 2 mM, what would be the resulting Kmapparent? [X]
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