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The table above shows the predicted results. A plus means when the strain with a mutation in the enzyme on the left is given radiolabeled A then radiolabel can be detected in the intermediate at the top. Are all the predicted results correct? yes no b) If you answered yes for part a, explain the results for row 3 (3 sentences MAX); if you answered no, state which boxes are wrong. c) Under what cellular conditions would you expect this pathway to be fully inhibited? (1 sentence Max) d) The upper branch (leading to F) is a pathway that is breaking down a metabolite to make ATP and the middle pathway uses ATP to synthesize I. How would you expect the following enzymes to be regulated by energy charge? Enzyme 1: Enzyme 4: Enzyme 6:

The table above shows the predicted results. A plus means when the strain with a mutation in the enzyme on the left is given radiolabeled A then radiolabel can be detected in the intermediate at the top. Are all the predicted results correct? yes no b) If you answered yes for part a, explain the results for row 3 (3 sentences MAX); if you answered no, state which boxes are wrong. c) Under what cellular conditions would you expect this pathway to be fully inhibited? (1 sentence Max) d) The upper branch (leading to F) is a pathway that is breaking down a metabolite to make ATP and the middle pathway uses ATP to synthesize I. How would you expect the following enzymes to be regulated by energy charge? Enzyme 1: Enzyme 4: Enzyme 6:

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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a) The table above shows the predicted results. A plus means when the strain with a mutation in the enzyme on the left is given radiolabeled A then radiolabel can be detected in the intermediate at the top. Are all the predicted results correct?

  1. yes
  2. no

b) If you answered yes for part a, explain the results for row 3 (3 sentences MAX); if you answered no, state which boxes are wrong.

c) Under what cellular conditions would you expect this pathway to be fully inhibited? (1 sentence Max)

d) The upper branch (leading to F) is a pathway that is breaking down a metabolite to make ATP and the middle pathway uses ATP to synthesize I. How would you expect the following enzymes to be regulated by energy charge?

Enzyme 1:

Enzyme 4:

Enzyme 6:

Below is a predicted branched pathway whose starting materials come from glycolysis. You conduct an experiment to confirm the pathway by growing mutants of all the enzymes on radiolabeled metabolite A. A 1 1234567 8 A + + + + + + + + _2 B B + + + + + + + с + + + + + + C D - + + + + + 4 3 E - + + + E 6、G7H8 + D F - + 5 + + + G - - + + + - LL + + F H + + + - - + I + + +
Transcribed Image Text:Below is a predicted branched pathway whose starting materials come from glycolysis. You conduct an experiment to confirm the pathway by growing mutants of all the enzymes on radiolabeled metabolite A. A 1 1234567 8 A + + + + + + + + _2 B B + + + + + + + с + + + + + + C D - + + + + + 4 3 E - + + + E 6、G7H8 + D F - + 5 + + + G - - + + + - LL + + F H + + + - - + I + + +
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