Equation 7a of the text,(Umax/KM1) [S] (v'max/KM2) [P]1+ [S]/KM1 + [P]/KM2-gives the expression for the rate of formation of product by a modified version of the Michaelis-Mentenmechanism in which the second step is also reversible. Derive the expression and find its limiting behaviour forhigh and low concentrations of substrate.

The following are given in the equation:V represents the rate of the reaction.S represents the…

Answered: Equation 7a of the text, (max/KM1)…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Similar questions

Conversion of F1,6BP to GA3P and DHAP by aldolase is striking in that it is strongly unfavorable when all species are at 1 M (∆G°’ = +22.8 kJ/mol) but favorable when the species are at their physiological conditions (∆G = -5.9 kJ/mol). Based on ∆G°’ and ∆G of aldolase and TIM, and using a reasonable physiological value of 15 mM for the concentration of F1,6BP, calculate the concentrations of GA3P and DHAP under physiological conditions (use T = 37°C). Why is the reaction so much more favorable under these conditions than when all species are at 1 M?
In a alkaline phosphatase kinetics experiments a studnet first made a standard curve with different amounts of PNP. In a reaction carried out with 10nM alkaline phosphatase and 100 uM PNPP substrate the student measured an absorbance value of 0.76 at 410nm after 30 seconds of reaction. Assuming the 30 second time point is in the lienar range of the reaction and the substrate has not been depleted at this time point, calculate the Vo at 100uM PNPP (in units of concentration per time).Beer's law formula given is y=0.0323x (y axis is Absorbance) (x Axis is PNP uM)
Since all of the B-lactams that you have tested are susceptible to hydrolysis by PAESBL-1, the hospital will not be able to use a single 8-lactam to combat this P. aeruginosa isolate. You need to consider alternative strategies. From the following list, select all of the strategies that might successfully overcome the broad substrate specificity PAESBL-1: Check all that apply. using multiple B-lactams simultaneously (e.g., rather than treating with amoxicillin alone, treat with the three B-lactams: amoxicillin, cephalothin, and imipenem). O using a reversible inhibitor of PAESBL-1 instead of a 8-lactam. Treat future infections by O combining a reversible inhibitor of PAESBL-1 with one or more 8-lactams. O using an irreversible inhibitor of PaESBL-1 instead of a B-lactam. O combining an irreversible inhibitor of PAESBL-1 with one or more -lactams. O O O O
Give a possible set of bacterial factors (or lack thereof) and what properties they have that would explain an increase in the 50% inhibitory concentration for tetracycline by 1,000-fold in an organism.
If the enzyme lactase has a Vo of 0.111111111111 mM per minute when [S] = 1.0 mM, and a Vo of 0.20 mM per minute when [S] = 5.0 mM, what is its Km? 0.125 mM 0.25 mM 0.50 mM 1.25 mM 5.0 mM
What is the role of the aspartate 118 residue in the Human inorganic Pyrophosphatase mechanism? include a drawing of the mechanistic proposal.

Recommended textbooks for you

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781305961135

Author:

Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Publisher:

Cengage Learning

Biochemistry

ISBN:

9781305577206

Author:

Reginald H. Garrett, Charles M. Grisham

Publisher:

Cengage Learning

Fundamentals of General, Organic, and Biological …

Biochemistry

ISBN:

9780134015187

Author:

John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson

Publisher:

PEARSON

SEE MORE TEXTBOOKS