In the "biochemical assay of b-galactosidase activity", what is o-nitrophenyl-b-D-galactosidase (ONPG) used for? O When ONPG is cleaved by b-galactosidase, we are able to assay b-galactosidase activity. O It provides essential nutrients for bacterial growth. O It partially disrupts the cell membrane to allow cellular proteins to diffuse out of the cell. O ONPG cleaves the b-galactosidase that is made by the lac operon so we can see how much activity there is in the cell. O ONPG cleaves galactose so we can measure how much lactose there is in the cell.
In the "biochemical assay of b-galactosidase activity", what is o-nitrophenyl-b-D-galactosidase (ONPG) used for? O When ONPG is cleaved by b-galactosidase, we are able to assay b-galactosidase activity. O It provides essential nutrients for bacterial growth. O It partially disrupts the cell membrane to allow cellular proteins to diffuse out of the cell. O ONPG cleaves the b-galactosidase that is made by the lac operon so we can see how much activity there is in the cell. O ONPG cleaves galactose so we can measure how much lactose there is in the cell.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
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Transcribed Image Text:In the "biochemical assay of b-galactosidase activity", what is o-nitrophenyl-b-D-galactosidase (ONPG)
used for?
O When ONPG is cleaved by b-galactosidase, we are able to assay b-galactosidase activity.
O It provides essential nutrients for bacterial growth.
It partially disrupts the cell membrane to allow cellular proteins to diffuse out of the cell.
O ONPG cleaves the b-galactosidase that is made by the lac operon so we can see how much activity there is
in the cell.
ONPG cleaves galactose so we can measure how much lactose there is in the cell.
Expert Solution
Step 1
Introduction
The lac operon model was first proposed by F. Jacob and J. Monod. Lac operon an operon system which comprises of three structural genes ( lac Z, lac Y, and lac A.) with a single promoter. The lac Z gene codes for beta-galactosidase, lac Y gene codes for permease and lac A codes for enzyme transacetylase.
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