serine proteases act via a two-step catalytic mechanism. However, as a critical scientist, you may want to see data that supports this claim. One experiment that can provide such evidence is called a 'pre-steady state' kinetics experiment using the chromogenic substrate, N-acetyl- phenylalanine p-nitrophenyl ester. As shown below when this substrate is cleaved, the p-nitrophenylate is a yellow product whose absorbance can be measured in real time using a spectrophotometer. When the chromogenic substrate is rapidly mixed together with a limiting amount of protease enzyme, the accumulation of product is measured right away on fast (millisecond) timescales. In this experiment, one observes two distinct phases of the reaction as shown in the plot below. Based upon what you know about the mechanism of serine proteases, provide a mechanistic interpretation for the observation of these two phases of the reaction. H₁C N-Acetyl-L-phenylalanine p-nitrophenyl ester Absorbance (ptrophenolate released + H₂O H₁₂C H₂C H Milliseconds after mixing p-Nitrophenolate
serine proteases act via a two-step catalytic mechanism. However, as a critical scientist, you may want to see data that supports this claim. One experiment that can provide such evidence is called a 'pre-steady state' kinetics experiment using the chromogenic substrate, N-acetyl- phenylalanine p-nitrophenyl ester. As shown below when this substrate is cleaved, the p-nitrophenylate is a yellow product whose absorbance can be measured in real time using a spectrophotometer. When the chromogenic substrate is rapidly mixed together with a limiting amount of protease enzyme, the accumulation of product is measured right away on fast (millisecond) timescales. In this experiment, one observes two distinct phases of the reaction as shown in the plot below. Based upon what you know about the mechanism of serine proteases, provide a mechanistic interpretation for the observation of these two phases of the reaction. H₁C N-Acetyl-L-phenylalanine p-nitrophenyl ester Absorbance (ptrophenolate released + H₂O H₁₂C H₂C H Milliseconds after mixing p-Nitrophenolate
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
![serine proteases act via a two-step catalytic mechanism. However, as a critical
scientist, you may want to see data that supports this claim. One experiment that can provide such
evidence is called a 'pre-steady state' kinetics experiment using the chromogenic substrate, N-acetyl-
phenylalanine p-nitrophenyl ester.
As shown below when this substrate is cleaved, the p-nitrophenylate is a yellow product whose
absorbance can be measured in real time using a spectrophotometer. When the chromogenic
substrate is rapidly mixed together with a limiting amount of protease enzyme, the accumulation of
product is measured right away on fast (millisecond) timescales. In this experiment, one observes two
distinct phases of the reaction as shown in the plot below. Based upon what you know about the
mechanism of serine proteases, provide a mechanistic interpretation for the observation of these two
phases of the reaction.
+ H₂O
N-Acetyl-L-phenylalanine p-nitrophenyl ester
p-Nitrophenolate
Absorbance
(p.nitrophenolate released
milliseconds after
mixing](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fd6957468-6fb1-4659-9083-987b06676d6d%2F7eeddbd3-f5cf-4f02-a2c6-2294c1d999de%2Fwaj04p_processed.png&w=3840&q=75)
Transcribed Image Text:serine proteases act via a two-step catalytic mechanism. However, as a critical
scientist, you may want to see data that supports this claim. One experiment that can provide such
evidence is called a 'pre-steady state' kinetics experiment using the chromogenic substrate, N-acetyl-
phenylalanine p-nitrophenyl ester.
As shown below when this substrate is cleaved, the p-nitrophenylate is a yellow product whose
absorbance can be measured in real time using a spectrophotometer. When the chromogenic
substrate is rapidly mixed together with a limiting amount of protease enzyme, the accumulation of
product is measured right away on fast (millisecond) timescales. In this experiment, one observes two
distinct phases of the reaction as shown in the plot below. Based upon what you know about the
mechanism of serine proteases, provide a mechanistic interpretation for the observation of these two
phases of the reaction.
+ H₂O
N-Acetyl-L-phenylalanine p-nitrophenyl ester
p-Nitrophenolate
Absorbance
(p.nitrophenolate released
milliseconds after
mixing
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