By analysis of the kinetics of interconversion of (1-13C]FBP and [6-13C]FBP and the appearance of 3-PGA and PEP in further experiments such as shown in the figure above, it was concluded that the rate of oxidation of glyceraldehyde-3-phosphate (GAP) was hindered under conditions of anaerobiosis. Separate experiments showed that the activity of 3-phosphoglcerate kinase was normaland that the concentrations of ADP and ATP were not limiting. What metabolic condition is the most likely to account for this conclusion? Write the reaction using structural formulas and naming reactants
By analysis of the kinetics of interconversion of (1-13C]FBP and [6-13C]FBP and the appearance of 3-PGA and PEP in further experiments such as shown in the figure above, it was concluded that the rate of oxidation of glyceraldehyde-3-phosphate (GAP) was hindered under conditions of anaerobiosis. Separate experiments showed that the activity of 3-phosphoglcerate kinase was normaland that the concentrations of ADP and ATP were not limiting. What metabolic condition is the most likely to account for this conclusion? Write the reaction using structural formulas and naming reactants
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
By analysis of the kinetics of interconversion of (1-13C]FBP and [6-13C]FBP and the appearance of 3-PGA and PEP in further experiments such as shown in the figure above, it was concluded that the rate of oxidation of glyceraldehyde-3-phosphate (GAP) was hindered under conditions of anaerobiosis. Separate experiments showed that the activity of 3-phosphoglcerate kinase was normaland that the concentrations of ADP and ATP were not limiting. What
![3. Glucose metabolism of nongrowing cells of
Lactococcus lactis MG5267 was monitored by
NMR under controlled conditions of pH, temper-
ature, and gas atmosphere with a time resolution
of 30 sec. The time course for glucose con-
sumption and product formation under anaerobic
conditions is shown in the figure on the right. As
indicated for the lower-most spectrum, upon ad-
dition of [1-13C]glucose, the resonance due to
lactate labeled on the methyl group was immedi-
ately observed; this resonance increased with
time until glucose was exhausted. (Abbrevia-
tions: PEP, phosphoenolpyruvate; 3-PGA, 3-
phosphoglycerate; [1-13C]FBP, (1-13C]fructose-
1,6-bis-phosphate; [6-13C]FBP, (6-1³C]fructose-
1,6-bis-phosphate; there are two resonances for
[1-13C]glucose, indicative of a- and B-anomers.)
Each spectrum was acquired over a time interval
of 30 sec. The numbers to the right of each spec-
trum indicate the time interval during which the
spectrum was acquired
[1.13C] Glucose
ppm
80
[1-17CFBP
ست
60
PEP
More in the
HAYA
wahamia
[6-13C]FBP
40
3-PGA
20
1.5-2
0.06 min
3-3.5
4.5-5
Lactate
7.5-8
19-19.5
95-10](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F3600976f-5a74-4553-80f1-af12fd18d682%2F45138d4c-8563-485e-b0bb-8ddeaa27c138%2Fn401gz_processed.png&w=3840&q=75)
Transcribed Image Text:3. Glucose metabolism of nongrowing cells of
Lactococcus lactis MG5267 was monitored by
NMR under controlled conditions of pH, temper-
ature, and gas atmosphere with a time resolution
of 30 sec. The time course for glucose con-
sumption and product formation under anaerobic
conditions is shown in the figure on the right. As
indicated for the lower-most spectrum, upon ad-
dition of [1-13C]glucose, the resonance due to
lactate labeled on the methyl group was immedi-
ately observed; this resonance increased with
time until glucose was exhausted. (Abbrevia-
tions: PEP, phosphoenolpyruvate; 3-PGA, 3-
phosphoglycerate; [1-13C]FBP, (1-13C]fructose-
1,6-bis-phosphate; [6-13C]FBP, (6-1³C]fructose-
1,6-bis-phosphate; there are two resonances for
[1-13C]glucose, indicative of a- and B-anomers.)
Each spectrum was acquired over a time interval
of 30 sec. The numbers to the right of each spec-
trum indicate the time interval during which the
spectrum was acquired
[1.13C] Glucose
ppm
80
[1-17CFBP
ست
60
PEP
More in the
HAYA
wahamia
[6-13C]FBP
40
3-PGA
20
1.5-2
0.06 min
3-3.5
4.5-5
Lactate
7.5-8
19-19.5
95-10
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