Homework Help is Here – Start Your Trial Now!
Science
Biochemistry
By analysis of the kinetics of interconversion of (1-13C]FBP and [6-13C]FBP and the appearance of 3-PGA and PEP in further experiments such as shown in the figure above, it was concluded that the rate of oxidation of glyceraldehyde-3-phosphate (GAP) was hindered under conditions of anaerobiosis. Separate experiments showed that the activity of 3-phosphoglcerate kinase was normaland that the concentrations of ADP and ATP were not limiting. What metabolic condition is the most likely to account for this conclusion? Write the reaction using structural formulas and naming reactants

By analysis of the kinetics of interconversion of (1-13C]FBP and [6-13C]FBP and the appearance of 3-PGA and PEP in further experiments such as shown in the figure above, it was concluded that the rate of oxidation of glyceraldehyde-3-phosphate (GAP) was hindered under conditions of anaerobiosis. Separate experiments showed that the activity of 3-phosphoglcerate kinase was normaland that the concentrations of ADP and ATP were not limiting. What metabolic condition is the most likely to account for this conclusion? Write the reaction using structural formulas and naming reactants

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
See similar textbooks
icon
Related questions
Question

 By analysis of the kinetics of interconversion of (1-13C]FBP and [6-13C]FBP and the appearance of 3-PGA and PEP in further experiments such as shown in the figure above, it was concluded that the rate of oxidation of glyceraldehyde-3-phosphate (GAP) was hindered under conditions of anaerobiosis. Separate experiments showed that the activity of 3-phosphoglcerate kinase was normaland that the concentrations of ADP and ATP were not limiting. What metabolic condition is the most likely to account for this conclusion? Write the reaction using structural formulas and naming reactants

3. Glucose metabolism of nongrowing cells of Lactococcus lactis MG5267 was monitored by NMR under controlled conditions of pH, temper- ature, and gas atmosphere with a time resolution of 30 sec. The time course for glucose con- sumption and product formation under anaerobic conditions is shown in the figure on the right. As indicated for the lower-most spectrum, upon ad- dition of [1-13C]glucose, the resonance due to lactate labeled on the methyl group was immedi- ately observed; this resonance increased with time until glucose was exhausted. (Abbrevia- tions: PEP, phosphoenolpyruvate; 3-PGA, 3- phosphoglycerate; [1-13C]FBP, (1-13C]fructose- 1,6-bis-phosphate; [6-13C]FBP, (6-1³C]fructose- 1,6-bis-phosphate; there are two resonances for [1-13C]glucose, indicative of a- and B-anomers.) Each spectrum was acquired over a time interval of 30 sec. The numbers to the right of each spec- trum indicate the time interval during which the spectrum was acquired [1.13C] Glucose ppm 80 [1-17CFBP ست 60 PEP More in the HAYA wahamia [6-13C]FBP 40 3-PGA 20 1.5-2 0.06 min 3-3.5 4.5-5 Lactate 7.5-8 19-19.5 95-10
Transcribed Image Text:3. Glucose metabolism of nongrowing cells of Lactococcus lactis MG5267 was monitored by NMR under controlled conditions of pH, temper- ature, and gas atmosphere with a time resolution of 30 sec. The time course for glucose con- sumption and product formation under anaerobic conditions is shown in the figure on the right. As indicated for the lower-most spectrum, upon ad- dition of [1-13C]glucose, the resonance due to lactate labeled on the methyl group was immedi- ately observed; this resonance increased with time until glucose was exhausted. (Abbrevia- tions: PEP, phosphoenolpyruvate; 3-PGA, 3- phosphoglycerate; [1-13C]FBP, (1-13C]fructose- 1,6-bis-phosphate; [6-13C]FBP, (6-1³C]fructose- 1,6-bis-phosphate; there are two resonances for [1-13C]glucose, indicative of a- and B-anomers.) Each spectrum was acquired over a time interval of 30 sec. The numbers to the right of each spec- trum indicate the time interval during which the spectrum was acquired [1.13C] Glucose ppm 80 [1-17CFBP ست 60 PEP More in the HAYA wahamia [6-13C]FBP 40 3-PGA 20 1.5-2 0.06 min 3-3.5 4.5-5 Lactate 7.5-8 19-19.5 95-10
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 3 steps with 1 images

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON
SEE MORE TEXTBOOKS