Look at the synthesis of citryl CoA by citrate synthase in the figure. Which of the 4 general catalytic mechanisms are present?Catalysis by approximation Covalent Catalysis General Acid-Base Catalysis Metal Ion Catalysis **Transcription for Educational Website:**### Diagram Explanation: Enzymatic Reaction PathwayThe diagram illustrates the enzymatic conversion process involving oxaloacetate and acetyl CoA to form a citryl CoA complex through an enol intermediate. The process is shown in three main stages, each represented by a molecular structure within an active site, highlighted in yellow.#### 1. Substrate Complex- **Components**: Oxaloacetate and Acetyl CoA- **Key Interactions**: - **Histidine 320** and **Aspartate 375**: These amino acid residues are positioned to facilitate the interaction. - **Histidine 274**: Interacts with the substrate complex, contributing to stabilization.- **Macromolecules**: - **Oxaloacetate**: Presented with carboxylate groups in blue. - **Acetyl CoA**: Highlighted in magenta.#### 2. Enol Intermediate- **Description**: Transition state between substrates and product.- **Key Reactions**: - Step **①** and **②** denote shifts in molecular structure aiding the progression. - **③** indicates the formation of the enol intermediate through enzyme-assisted protonations and deprotonations.#### 3. Citryl CoA Complex- **Resulting Product**: Formation of Citryl CoA.- **Structure**: - Combines previous molecular structures with key interactions via the amino acids.- **Final Arrangement**: Stabilized by the active site residues, indicating successful transformation from reactants to a complex product.The diagram provides a step-by-step visual understanding of the enzyme-catalyzed reaction, emphasizing the role of specific amino acids and the structural changes leading to product formation.

Citrate synthase is an important enzyme in citric acid cycle that catalyses the conversion of oxalo…

Look at the synthesis of citryl CoA by citrate synthase in the figure. Which of the 4 general catalytic mechanisms are present? Catalysis by approximation Covalent Catalysis General Acid-Base Catalysis Metal Ion Catalysis

Look at the synthesis of citryl CoA by citrate synthase in the figure. Which of the 4 general catalytic mechanisms are present? Catalysis by approximation Covalent Catalysis General Acid-Base Catalysis Metal Ion Catalysis

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

Look at the synthesis of citryl CoA by citrate synthase in the figure. Which of the 4 general catalytic mechanisms are present?

Catalysis by approximation

Covalent Catalysis

General Acid-Base Catalysis

Metal Ion Catalysis

**Transcription for Educational Website:**

### Diagram Explanation: Enzymatic Reaction Pathway

The diagram illustrates the enzymatic conversion process involving oxaloacetate and acetyl CoA to form a citryl CoA complex through an enol intermediate. The process is shown in three main stages, each represented by a molecular structure within an active site, highlighted in yellow.

#### 1. Substrate Complex
- **Components**: Oxaloacetate and Acetyl CoA
- **Key Interactions**:
- **Histidine 320** and **Aspartate 375**: These amino acid residues are positioned to facilitate the interaction.
- **Histidine 274**: Interacts with the substrate complex, contributing to stabilization.
- **Macromolecules**:
- **Oxaloacetate**: Presented with carboxylate groups in blue.
- **Acetyl CoA**: Highlighted in magenta.

#### 2. Enol Intermediate
- **Description**: Transition state between substrates and product.
- **Key Reactions**:
- Step **①** and **②** denote shifts in molecular structure aiding the progression.
- **③** indicates the formation of the enol intermediate through enzyme-assisted protonations and deprotonations.

#### 3. Citryl CoA Complex
- **Resulting Product**: Formation of Citryl CoA.
- **Structure**:
- Combines previous molecular structures with key interactions via the amino acids.
- **Final Arrangement**: Stabilized by the active site residues, indicating successful transformation from reactants to a complex product.

The diagram provides a step-by-step visual understanding of the enzyme-catalyzed reaction, emphasizing the role of specific amino acids and the structural changes leading to product formation.

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