If you can clearly visualize the chymotrypsin mechanism of action, you should be able to picture the structure of the transition state right after the enzyme attacks the first substrate. Think hard about what we have covered, and visualize that transition state accurately:

Human Anatomy & Physiology (11th Edition)
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Chapter1: The Human Body: An Orientation
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**Visualizing the Chymotrypsin Mechanism of Action**

To understand the chymotrypsin mechanism of action, it is crucial to visualize the structure of the transition state that occurs just after the enzyme attacks the first substrate. Reflect on the material we have covered and try to accurately picture that transition state.

**Central Carbon in the Tetrahedral Transition State**

The central carbon in the tetrahedral transition state forms covalent links with multiple atoms and groups, but not all. Consider the following options and determine which among them the central carbon is not directly covalently linked to.

Which one is the central carbon not **directly** covalently linked to? Choose the best answer:

- The R-group that is in the specificity pocket.
- The nitrogen of the peptide bond.
- The strong nucleophile from the enzyme's catalytic site.
- The carbonyl oxygen of the peptide bond.
Transcribed Image Text:**Visualizing the Chymotrypsin Mechanism of Action** To understand the chymotrypsin mechanism of action, it is crucial to visualize the structure of the transition state that occurs just after the enzyme attacks the first substrate. Reflect on the material we have covered and try to accurately picture that transition state. **Central Carbon in the Tetrahedral Transition State** The central carbon in the tetrahedral transition state forms covalent links with multiple atoms and groups, but not all. Consider the following options and determine which among them the central carbon is not directly covalently linked to. Which one is the central carbon not **directly** covalently linked to? Choose the best answer: - The R-group that is in the specificity pocket. - The nitrogen of the peptide bond. - The strong nucleophile from the enzyme's catalytic site. - The carbonyl oxygen of the peptide bond.
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