### Genetic Activity of the lac Operon under Various Cellular Conditions**Instructions:**Predict the level of genetic activity of the *lac operon* as well as the status of the lac repressor and the CAP protein under the following cellular conditions (indicated by the presence (+) or absence (-) of lactose and glucose).### Cellular Conditions Table| | Lactose | Glucose ||---|---------|---------|| a) | – | – || b) | + | – || c) | – | + || d) | + | + |### Prediction QuestionsFor each set of conditions, answer the following questions:#### a) - Operon (on or off or basal?)- Lac repressor (bound to operator?)- CAP protein (bound to CAP site?)#### b)- Operon (on or off or basal?)- Lac repressor (bound to operator?)- CAP protein (bound to CAP site?)#### c)- Operon (on or off or basal?)- Lac repressor (bound to operator?)- CAP protein (bound to CAP site?)#### d)- Operon (on or off or basal?)- Lac repressor (bound to operator?)- CAP protein (bound to CAP site?)

In lac operon, structural genes responsible for lactose metabolism are all under the control of a…

Answered: 4. Predict the level of genetic…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Given the Table below concerning the lac operon, match the letter with what would be occurring for each scenario. Repressor is bound to the operator, cAMP complex is bound and transcription is prevented. Allolactose is bound to the repressor, cAMP complex is bound, and transcription is prevalent. Allolactose is bound to the repressor, cAMP complex is not assembled, and transcription is slow Repressor is bound to the operator, cAMP complex is not assembled, and transcription is prevented.
1. The lactose operon is controlled by both lactose and glucose. Fill in the following table to indicate what would be occurring under different cellular conditions Lactose absent present absent present Glucose Operon Activity (high/low) absent absent present present low high 10W high CAMP Repressor (bound/free) (high/low) bound free buind will high high low CAP (bound/free) bound free full
Explain how the lac operon is regulated under the following conditions. Include the following terms in your answers: Repressor, Operator, RNA polymerase, CAP site, CAP protein, Promoter A. Lactose and glucose are both present. B. Lactose and glucose are both absent. C. Lactose is present and glucose is absent.
1. Please tell me: What's a mutation in moleculer terms? 2. When a mutation deletes a base in genomic DNA, how does it affect the expression product production and reading frame? 3. How does induction control enzyme synthesis using lactose operon?
For a lac operon of genotype l'POʻ*, functional beta-galactosidase is ... (pick the best answer) Select one: O a. Never produced O b. Constitutively produced O c. Under inducible control
28. Which statement does NOT explain why the lac operon would not be effectively regulated by an attenuation mechanism like the trp operon? 1.Lactose is a substrate for the lac operon protein products. 2.Products of lac operon enzymes are not used in protein synthesis. 3.Transcription and translation do not happen simultaneously for the lac operon. 4.Attenuation is a suitable regulatory mechanism for anabolic amino acid pathways. 5.All of these statements explain why regulation by attenuation would not be effective for the lac operon.
Like the lac operon, the hexose operon is controlled by a separate regulatory protein under the control of its own promoter (see the schematic of the operon below). The hexose regulatory protein is sensitive to fatty acyl CoA levels. When all hexose fuel sources are depleted, the bacteria switch to lipid metabolism and fatty acyl CoA levels increase. This turns expression of the hexose operon off. +1 +1 Regulatory Gene Pregulator P operon Hexose Operon Genes regulator promoter operon promoter
. Listed in parts a through g are some mutations that were found in the 5′ UTR of the trp operon of E. coli. What will the most likely effect of each of these mutations be on the transcription of the trp structural genes? Q. A mutation that prevents the binding of the ribosome to the 5′ end of the mRNA 5′ UTR
Match each description with the appropriate label (allo)Lactose binds to the lac repressor, causing it to release from the promoter CAP binds CAMP to stimulate transcripti of the lac operon ✓ [Choose ] positive control of an inducible gene negative control of an inducible gene [Choose]

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