1. The lactose operon is controlled by both lactose and glucose. Fill in the following table toindicate what would be occurring under different cellular conditionsLactoseabsentpresentabsentpresentGlucose OperonActivity(high/low)absentabsentpresentpresentlowhigh10WhighCAMPRepressor(bound/free) (high/low)boundfreebuindwillhighhighlowCAP(bound/free)boundfreefull

The lactose operon is a catabolic operon in Ecoli that encodes proteins that are necessary for the…

Answered: 1. The lactose operon is controlled by…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Given the Table below concerning the lac operon, match the letter with what would be occurring for each scenario. Repressor is bound to the operator, cAMP complex is bound and transcription is prevented. Allolactose is bound to the repressor, cAMP complex is bound, and transcription is prevalent. Allolactose is bound to the repressor, cAMP complex is not assembled, and transcription is slow Repressor is bound to the operator, cAMP complex is not assembled, and transcription is prevented.
2. Assume you have identified a new operon in bacteria (which you call the suc operon) that encodes enzymes required for catabolism of the sugar sucrose. The structural genes that are required to breakdown sucrose are transcribed from a promoter (Psuc). One of these enzymes is encoded by the sucS gene. Expression of the operon is controlled by a regulator, called SucR, which binds to an operator (0). Using your knowledge of the lac operon, interpret the results of the following experiments (Table 1) to propose a model describing how the sucrose operon might be regulated. Cell genotype Production of SucS enzyme Without sucrose With sucrose R* o* p* s* R O* p* s* R* O P* s* ++ -- -- -- --- -- RO* p* s* / R* o* p* s R° O* p* s ++ -- ++ ++ Table 1. Production of SucS enzyme in various strains. "-" in the cell genotype indicates a loss of function mutation, "+" indicates wild type function, two genotypes separated by a/ indicate a merodiploid or partial diploid. R° is a mutant version of…
The operator of the lactose operon in E. Coli is located: between the promoter and the repressor gene upstream of the promoter between the promoter and the structural genes anywhere on the chromosome among the structural genes
Give typing answer with explanation and conclusion
The lac operon produces: (choose the best answer) OA single mRNA molecule O A single protein OA single mRNA molecule and a single protein O Multiple proteins OA single mRNA molecule and multiple proteins
In the absence of lactose and the presence of glucose the lac operon is strongly expressed Lacl is not bound bound to the CAP binding site. to the operator and CAP is
This figure represents the ABC operon, which is a negative inducible operon, and its associated regulatory gene. The structural genes produced by the operon convert molecule A into molecule D. When molecule A is present in the environment, it will bind to the regulatory protein, and operon expression will be induced. operator testR testR promoter regulatory protein testP testo structural genes test1 test1 test2 test2 test3 test3 structural proteins
This figure represents the ABC operon, which is a negative inducible operon, and its associated regulatory gene. The structural genes produced by the operon convert molecule A into molecule D. When molecule A is present in the environment, it will bind to the regulatory protein, and operon expression will be induced. operator testR testR promoter regulatory protein testP testo structural genes test1 test1 test2 test2 test3 test3 structural proteins
1. (a) Lactose levels are high Lac Operon Genes are switched OFF Lac Operon Genes are switched ON Does NOT affect the gene expression (b) Enhancer and activator proteins are working together Gene is switched ON Gene is switched OFF Does NOT affect the gene expression
CAP-binding site Gene for Operator Gene for repressor protein Promoter for I gene Promoter for permease lac operon Gene for Gene for B-galactosidase transacetylase lac P, A Regulatory region Coding region For the above picture, draw all the proteins and molecules AND WHERE THEY BIND under conditions of no lactose, no glucose.
28. Which statement does NOT explain why the lac operon would not be effectively regulated by an attenuation mechanism like the trp operon? 1.Lactose is a substrate for the lac operon protein products. 2.Products of lac operon enzymes are not used in protein synthesis. 3.Transcription and translation do not happen simultaneously for the lac operon. 4.Attenuation is a suitable regulatory mechanism for anabolic amino acid pathways. 5.All of these statements explain why regulation by attenuation would not be effective for the lac operon.
When lactose is present lactose induces the activation of the genes in the lac operon by binding the repressor lactose represses the activation of the genes in the lac operon by binding the repressor lactose induces the activation of the genes in the lac operon by binding the operator lactose represses the activation of the genes in the lac operon by binding the operator

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