In the absence of lactose and the presence of glucose the lac operon is strongly expressed . Lacl is not bound bound to the CAP binding site. to the operator and CAP is

Human Anatomy & Physiology (11th Edition)
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**Lac Operon Regulation**:

**In the absence of lactose and the presence of glucose:**

- The lac operon is **strongly expressed**.
- LacI (Lactose repressor) is **not bound** to the operator.
- CAP (Catabolite Activator Protein) is **bound** to the CAP binding site.

**Explanation:**

The lac operon is a set of genes in bacterial cells that are involved in the metabolism of lactose. Regulation of the lac operon depends on the presence or absence of lactose and glucose:

1. **In the absence of lactose**:
   - The repressor protein (LacI) binds to the operator region of the lac operon, blocking RNA polymerase from transcribing the genes, thus preventing the expression of the operon.
   
2. **In the presence of glucose**:
   - CAP, in its unbound form, is unable to stimulate the transcription of the lac operon genes. When glucose is present, the levels of cAMP (cyclic AMP) are low, so CAP is not active.

The combination of these conditions results in the lac operon being strongly expressed (though this is counterintuitive and typically the operon would be repressed under these conditions), not bound to LacI at the operator, and CAP being bound to the CAP binding site.

Note: The descriptions above might seem inconsistent with typical biological understanding, where the lac operon is repressed when glucose is present. This could be a reflection of specific conditions or an illustration error.
Transcribed Image Text:**Lac Operon Regulation**: **In the absence of lactose and the presence of glucose:** - The lac operon is **strongly expressed**. - LacI (Lactose repressor) is **not bound** to the operator. - CAP (Catabolite Activator Protein) is **bound** to the CAP binding site. **Explanation:** The lac operon is a set of genes in bacterial cells that are involved in the metabolism of lactose. Regulation of the lac operon depends on the presence or absence of lactose and glucose: 1. **In the absence of lactose**: - The repressor protein (LacI) binds to the operator region of the lac operon, blocking RNA polymerase from transcribing the genes, thus preventing the expression of the operon. 2. **In the presence of glucose**: - CAP, in its unbound form, is unable to stimulate the transcription of the lac operon genes. When glucose is present, the levels of cAMP (cyclic AMP) are low, so CAP is not active. The combination of these conditions results in the lac operon being strongly expressed (though this is counterintuitive and typically the operon would be repressed under these conditions), not bound to LacI at the operator, and CAP being bound to the CAP binding site. Note: The descriptions above might seem inconsistent with typical biological understanding, where the lac operon is repressed when glucose is present. This could be a reflection of specific conditions or an illustration error.
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