Predict the level of genetic activity of the lac operon as well as the status of the lac repressor and the CAP protein under the cellular conditions listed in the following table (+ = sugar present; = sugar absent): Cellular conditions Lactose Glucose + + + a) Operon (on or off or basal?) b) Operon (on or off or basal?) Lac repressor (bound to operator?) CAP protein (bound to CAP site?) d) Operon (on or off or basal?) PROO Lac repressor (bound to operator?) CAP protein (bound to CAP site?) c) Operon (on or off or basal?) Lac repressor (bound to operator?) CAP protein (bound to CAP site?) Lac repressor (bound to operator?) CAP protein (bound to CAP site?)
Predict the level of genetic activity of the lac operon as well as the status of the lac repressor and the CAP protein under the cellular conditions listed in the following table (+ = sugar present; = sugar absent): Cellular conditions Lactose Glucose + + + a) Operon (on or off or basal?) b) Operon (on or off or basal?) Lac repressor (bound to operator?) CAP protein (bound to CAP site?) d) Operon (on or off or basal?) PROO Lac repressor (bound to operator?) CAP protein (bound to CAP site?) c) Operon (on or off or basal?) Lac repressor (bound to operator?) CAP protein (bound to CAP site?) Lac repressor (bound to operator?) CAP protein (bound to CAP site?)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
Related questions
Concept explainers
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Question
Transcribed Image Text:**Predict the Level of Genetic Activity of the lac Operon**
In this educational section, we aim to predict the level of genetic activity of the lac operon, along with the status of the lac repressor and the CAP protein under various cellular conditions. The table below indicates the presence (+) or absence (−) of lactose and glucose in the cellular environment:
| Cellular Conditions | Lactose | Glucose |
|:---------------------:|:--------:|:--------:|
| a) | − | − |
| b) | + | − |
| c) | − | + |
| d) | + | + |
For each of the conditions (a, b, c, and d), we need to determine the following:
- Whether the operon is on, off, or at a basal level of activity?
- Is the lac repressor bound to the operator?
- Is the CAP protein bound to the CAP site?
### Detailed Explanations:
#### Condition a)
- **Operon:** On or off or basal?
- **Lac Repressor:** Bound to operator?
- **CAP Protein:** Bound to CAP site?
#### Condition b)
- **Operon:** On or off or basal?
- **Lac Repressor:** Bound to operator?
- **CAP Protein:** Bound to CAP site?
#### Condition c)
- **Operon:** On or off or basal?
- **Lac Repressor:** Bound to operator?
- **CAP Protein:** Bound to CAP site?
#### Condition d)
- **Operon:** On or off or basal?
- **Lac Repressor:** Bound to operator?
- **CAP Protein:** Bound to CAP site?
**Explanation:**
1. **Lactose (−) and Glucose (−)**: No lactose to induce the operon and low glucose leading to active CAP protein due to high cAMP levels.
2. **Lactose (+) and Glucose (−)**: Lactose present to induce the operon and absence of glucose will activate CAP protein.
3. **Lactose (−) and Glucose (+)**: No lactose to induce the operon and glucose presence will inactivate CAP protein.
4. **Lactose (+) and Glucose (+)**: Lactose present to induce the operon but high glucose will suppress CAP protein binding due to low cAMP levels.
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
Step by step
Solved in 2 steps
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education