Suppose you cultured yeast, that you had transformed with PMZ379 plus the AVT5 SGRNA, on a plate of YES agar plus salt ions. You then cultured untransformed yeast on a plate containing YES agar plus salt ions. Your results showed growth on both plates. Which of the following is the most reasonable interpretation of your results? Question options: A)The untransformed yeast spontaneously mutated to have a Čas9 gene that could silence the AVT5 gene. B)The yeast had a mutation in the AVT5 gene that made the protein non-functional. C)The sgRNÁ gene correctly guided the Cas9 protein to the AVT5 gene to silence it in the transformed yeast. D)The conditions for the experiment were so exceptional, that they promoted growth in both the modified and unmodified yeast.

Human Anatomy & Physiology (11th Edition)
11th Edition
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Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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Suppose you cultured yeast, that you had
transformed with PMZ379 plus the AVT5 SGRNA,
on a plate of YES agar plus salt ions. You then
cultured untransformed yeast on a plate
containing YES agar plus salt ions. Your results
showed growth on both plates. Which of the
following is the most reasonable interpretation of
your results?
Question options:
A)The untransformed yeast spontaneously
mutated to have a Čas9 gene that could silence
the AVT5 gene.
B)The yeast had a mutation in the AVT5 gene that
made the protein non-functional.
C)The sgRNA gene correctly guided the Cas9
protein to the AVT5 gene to silence it in the
transformed yeast.
D)The conditions for the experiment were so
exceptional, that they promoted growth in both
the modified and unmodified yeast.
Transcribed Image Text:Suppose you cultured yeast, that you had transformed with PMZ379 plus the AVT5 SGRNA, on a plate of YES agar plus salt ions. You then cultured untransformed yeast on a plate containing YES agar plus salt ions. Your results showed growth on both plates. Which of the following is the most reasonable interpretation of your results? Question options: A)The untransformed yeast spontaneously mutated to have a Čas9 gene that could silence the AVT5 gene. B)The yeast had a mutation in the AVT5 gene that made the protein non-functional. C)The sgRNA gene correctly guided the Cas9 protein to the AVT5 gene to silence it in the transformed yeast. D)The conditions for the experiment were so exceptional, that they promoted growth in both the modified and unmodified yeast.
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