Control - Rat liver Suspected mutagen Rat liver extract extract Salmonella- strain (requires histidine) -Salmonella strain (requires histidine) Plate the mixtures onto petri plates that lack histidine. Incubate overnight to allow bacterial growth. A large number of colonies suggests that the suspected mutagen causes mutation. FIGURE 19.15 The Ames test for mutagenicity.

Q: Why are the correct primer sequences essential for successful amplification?

A: Polymerase chain reaction (PCR) is a widely used method in molecular biology that is used to make…

Q: Why is the restriction enzyme digestion step critical in 3C assays?

A: Genetic information is stored in DNA and we often consider the DNA molecule only as a linear…

Q: Using the restriction site for SalI, suggest another restriction enzyme that would cut at the same…

A: Isoschizomers are a set of enzymes that share the same recognition sequence in the restriction…

Q: question 2. To detect trisomy 18 in amniotic fluid cells the most optimal probe type to use is A)…

A: Trisomy 18, also known as Edwards syndrome, is a genetic disorder that is caused by the presence of…

Q: From what the chipko andolan was originated?

A: Chipko Andolab also known as chipko (means — hug) movement. This was nonviolent social ecological…

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A: Restriction enzymes are a group of enzymes that can cut double-stranded DNA. They are endonucleases…

Q: What are nanopores used for and tell How does DNA bind to the nanopore?

A: Deoxyribonucleic acid (DNA) sequencing is the process of determining the nucleic acid sequence in…

Q: What are the reagents found in a restriction enzyme digestion buffer? What are the roles of these…

A: Restriction enzymes are enzymes that recognise and cleave DNA at or near specific DNA sequences.…

Q: What does it mean if a restriction enzyme produces ‘sticky’ or ‘blunt’ ends? Does the restriction…

A: The sticky or blunt ends refers to the edges of DNA molecules. The sticky ends tend to overlap to…

Q: What is the function of a restriction enzyme?

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Q: What is the Ti plasmid and how has it been of use ingenetic engineering?

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Q: Name the technique used for amplification of DNA?

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A: The chain elongating inhibitors of the DNA polymerase which are required for the Sanger method of…