The Cas9 enzyme requires a short sequence called a PAM (Protospacer Adjacent Motif) for cleaving the target DNA.This PAM sequence is not found in the CRISPR locus of Streptococcus pyogenes but it is present in viral genomes and we need to make sure it is present adjacent to where the guide RNA binds the yeast DNA. What does this suggest about the function of the PAM?

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