A eukaryotic cDNA was ligated to a phage DNA which was transfected into a bacterial host cell. The host then transcribed and translated it. The protein synthesized however, was determined to be non-functional. What is the MOST probable reason for this defect? mutation in the cDNA O absence of post-transcriptional modification in bacteria O differences in post-translational modification in bacteria and eukaryotes error in the addition of amino acids by the aminoacyl synthetase
A eukaryotic cDNA was ligated to a phage DNA which was transfected into a bacterial host cell. The host then transcribed and translated it. The protein synthesized however, was determined to be non-functional. What is the MOST probable reason for this defect? mutation in the cDNA O absence of post-transcriptional modification in bacteria O differences in post-translational modification in bacteria and eukaryotes error in the addition of amino acids by the aminoacyl synthetase
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
Question
Transcribed Image Text:A eukaryotic cDNA was ligated to a phage DNA
which was transfected into a bacterial host cell.
The host then transcribed and translated it. The
protein synthesized however, was determined to be
non-functional. What is the MOST probable reason
for this defect?
mutation in the cDNA
O absence of post-transcriptional modification in
bacteria
O differences in post-translational modification in
bacteria and eukaryotes
error in the addition of amino acids by the aminoacyl
synthetase
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