A eukaryotic cDNA was ligated to a phage DNA which was transfected into a bacterial host cell. The host then transcribed and translated it. The protein synthesized however, was determined to be non-functional. What is the MOST probable reason for this defect? mutation in the cDNA O absence of post-transcriptional modification in bacteria O differences in post-translational modification in bacteria and eukaryotes error in the addition of amino acids by the aminoacyl synthetase

Q: The trp repressor binds to the operator region of DNA as a dimer (two repressor protein subunits…

A: The trp operon regulation is a repressible system. When the effector molecule binds to the…

Q: Unlike transcription in most prokaryotes, transcription in eukaryotes Group of answer choices makes…

A: Transcription is a molecular process in which DNA is transcribed into mRNA. This process occurs both…

Q: O mediator complex O histones RNA polymerase enhancer

A: Transcription is the process by which the information of a strand on the DNA is replicated by its…

Q: What are the functional consequences of this deletion for lilP mRNA transcription and translation?…

A: Introduction :- A deletion mutation is a type of genetic mutation that involves the loss of one or…

Q: The data below depicts the binding of the E coli RNA Polymerase to DNA containing a promoter…

A: RNA polymerase is specific enzyme that synthesized RNA from the strand of DNA. They helps to copying…

Q: The trpL sequence in the trp operon encodes O a poly-A tail addition sequence O rho termination…

A: Answer :- several codons for the amino acid trp  Because the in the  E. coli operon that is the…

Q: Transcription start site selection by S. cerevisiae Pol II occurs over a range of positions located…

A: Introduction: Transcription start sites (TSSs) are acquired by RNA polymerase II (Pol II) in…

Q: describe the process of transcription of DNA based off of these figure

A: Transcription is the process of the formation of RNA copy from the DNA. The main enzyme responsible…

Q: Some prion-related diseases, such as familial fatal insomnia, areinherited. How would you expect the…

A: Familial fatal insomnia is a medical condition characterized by insomnia or trouble sleeping. This…

Q: Here is a list of knockout gene mutations that are desired to make in E. coli genome. You should…

A: A number of knockout gene mutations in the E. coli genome are shown to us in this scenario. Each…

Q: If this was an Eukaryote, after this transcript undergoes processing and exits the nucleus what…

A: The question is asking about the structure of a mature mRNA transcript in a eukaryotic cell after it…

Q: radioactively labeled cDNA is used to probe RNA isolated from kidney by Northern blotting. Two bands…

A: Northern blotting is a laboratory technique used to detect and analyze specific RNA molecules. It…

Q: What do the promoters of nearly all eukaryotic genes contain?

A: Promoter is a region in a gene where enhancer binds and transcription of gene starts.     TATA…

Q: Which of the following DNA regions Is NOT Involved in gene expressi regulation in eukaryotes?

A: Ans- Operators are not involved in the Eukaryotic Gene Expression. Eukaryotic protein-coding genes…

Q: Please answer the following question

A: Hope it help answer your questions. Let me know if you have any clarification. Thank you.

Q: In transcription Rho-dependent termination in bacteriophage is more common than in bacteria. True…

A: Transcription is defined as the synthesis of mRNA from the template strand of DNA. The RNA…

Q: 20 ORF for gene X e for gene X above has a hypomorphic mutation (which you name Mutation #1) that is…

A: Answer.) :-The given question presents an experimental scenario involving Gene X, which has a…

Q: Pre-initiation complex (PIC) is formed right before the initiation of genes, which of the following…

A: The preinitiation complex is a protein construct that forms in eukaryotic cells prior to…

Q: You are studying the ABCD operon from a newly isolated bacterium. Which of the following would…

A: A Operon consists of segments of DNA , which carry codes for the synthesis of proteins. These genes…

Q: You have cloned a novel E. coli gene and wish to express protein from it in human cells. In order to…

A: The Central Dogma theory explains that DNA forms DNA by Replication, DNA forms RNA by Transcription…

Q: You have created a deletion of the ribosome binding site for the leader sequence of the trp operon.…

A: trp stands for tryptophan operon.In trp operon, there are 5 structural genes viz., trp E, trpD,…

Q: A mutation in the trp repressor gene that prevents the apo-repressor from binding the co-repressor…

A: Tryptophan operon Tryptophan operon is negatively regulated operon, it includes five gene, promotor,…

Q: explain this image for the ecoli bacteria mutation trpE

A: Introduction The trp operon is a type of operon system found in E. coli bacteria. The trp operon…

Q: You have isolated a mutant that you call Mutant Z. In a Mutant Z strain, the RNA polymerase can bind…

A: Here, the correct answer is option c) domain 2 of sigma and d) 3/4 linker of sigma.

Q: Usually found in prokaryotes, regulatory sequences lying adjacent to the DNA being transcribed are…

A: In prokaryotes, the process of transcription and translation occur simultaneously due to the lack of…

Q: You have replaced all of the tryptophan codons in the trp leader sequence with leucine codons. If…

A: A codon is a sequence of three nucleotides (DNA or RNA) that corresponds to a specific amino acid or…

Q: According to the operon hypothesis, the Z and Y genes of E. coli are classified as structural genes,…

A: The Lac Operon is responsible for Lactose metabolism and transport in E.coli. This operon consists…

Q: Match the following (most appropriate combinations): Alpha-amanitin Sigma 70 Sigma 54 -35…

A: Ocean urchin, rodent liver, and calf thymus alpha-amanitin are potent inhibitors of DNA-subordinate…

Q: Which of the following is a likely outcome of Rifampin treatment? Transcriptional elongation is…

A: Answer. Rifampicin is a major derivative of the antibiotic rifamycin. This antibiotic binds to the…

Q: Assuming that the level of glucose is low, a mutation in the repressor associated with the lac…

A: The lac operon which is also known as lactose operon is a set of genes that are specific for uptake…

Proteins

We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.  

Protein Expression

The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.