describe the process of transcription of DNA based off of these figure

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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describe the process of transcription of DNA based off of these figure

If the RNA polymerase followed the template strand around the axis of the DNA duplex,
there would be no strain, and no supercoiling of the DNA would occur, but the RNA chain
would be wrapped around the double helix once every 10 base pairs. This possibility seems
unlikely because it would be difficult to disentangle the transcript from the DNA complex.
RNA
RNA
polymerase
Торoisomerase
removing negative
supercoil
Topoisomerase
removing positive
supercoil
RNA
polymerase
Alternatively, topoisomerases coukd remove the supercoils. A topoisomerase capable of relaxing
positive supercoils situated ahead of the advancing transcription bubble would "relax" the DNA.
A second topoisomerase behind the bubble would remove the negative supercoils.
Transcribed Image Text:If the RNA polymerase followed the template strand around the axis of the DNA duplex, there would be no strain, and no supercoiling of the DNA would occur, but the RNA chain would be wrapped around the double helix once every 10 base pairs. This possibility seems unlikely because it would be difficult to disentangle the transcript from the DNA complex. RNA RNA polymerase Торoisomerase removing negative supercoil Topoisomerase removing positive supercoil RNA polymerase Alternatively, topoisomerases coukd remove the supercoils. A topoisomerase capable of relaxing positive supercoils situated ahead of the advancing transcription bubble would "relax" the DNA. A second topoisomerase behind the bubble would remove the negative supercoils.
RNA
pol
Step 1 Recognition of promoter
by o; binding of polymerase
holoenzyme to DNÁ;
migration to promoter
5'
Promoter
3'
5'
DNA template
Step 2 Formation of an RNA
5'
polymerase:closed promoter 3t
complex
Step 3 Unwinding of DNA at
promoter and formation
3
of open promoter complex
Purine NTP
Step 4 RNA polymerase initiates
mRNA synthesis, almost
always with a purine
5'
3'
5'
PPP Fa
NTPS
Step 5 RNA polymerase holoenzyme- 3'[
catalyzed elongation of
MRNA by about four more
nucleotides
3'
PPP PuNINININA
Step 6 Release of o-subunit as core
RNA polymerase proceeds
down the template, elongating
RNA transcript
Promoter
3'
P P P Pu NNNNI
5'
in io
o io
e io
Transcribed Image Text:RNA pol Step 1 Recognition of promoter by o; binding of polymerase holoenzyme to DNÁ; migration to promoter 5' Promoter 3' 5' DNA template Step 2 Formation of an RNA 5' polymerase:closed promoter 3t complex Step 3 Unwinding of DNA at promoter and formation 3 of open promoter complex Purine NTP Step 4 RNA polymerase initiates mRNA synthesis, almost always with a purine 5' 3' 5' PPP Fa NTPS Step 5 RNA polymerase holoenzyme- 3'[ catalyzed elongation of MRNA by about four more nucleotides 3' PPP PuNINININA Step 6 Release of o-subunit as core RNA polymerase proceeds down the template, elongating RNA transcript Promoter 3' P P P Pu NNNNI 5' in io o io e io
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