Which of the two restriction sites in pQE vector will be appropriate to allow full length insertion of your gene? These restriction sites will serve as linkers on both ends of your gene

Q: The tac promoter, an artificial promoter made from portions of the frp and lacUV5 promoters, has…

A: The lac operon is an operon required for the transport and metabolism of lactose in E.coli and many…

Q: You are studying a human gene, and try to express the protein in E. coli bacterial cells. To do…

A: Cloning is the process of creating a genetically identical copy of a biological entity. Cloning can…

Q: As a Master's student in a lab you are given a plasmid vector that has the lacZ operon system and a…

A: INTRODUCTION - Insert recombinant DNA into host cell is called as Plasmid vector. EXPLANATION - STEP…

Q: You are interested in studying resistance to heavy metals and have selected the yeast Saccharomyces…

A: (a) The advantage of using a deletion mutant such as pgz-1 is that it allows for the identification…

Q: A full-length eukaryotic gene is inserted into a bacterial chromosome. The gene contains a complete…

A: The eukaryotic DNA is composed of coding and non-coding sequences, almost 90% are non-coding…

Q: Here is a list of knockout gene mutations that are desired to make in E. coli genome. You should…

A: A number of knockout gene mutations in the E. coli genome are shown to us in this scenario. Each…

Q: What is a consensus sequence? How similar to the consensus sequence would you expect the promoter of…

A: Introduction  An area of DNA upstream of a gene called a promoter, as it relates to genomics, is…

Q: Explain the difference in results for the RT-PCR from the CD4+ T cells and the RT-PCR from the CD8+…

A: RT-PCR (Reverse Transcription Polymerase Chain Reaction) is a molecular technique commonly used to…

Q: What would be the consequence of inserting 10 random base pairs immediately downstream of the -10…

A: The insertion of 10 random base pairs immediately downstream of the -10 box in a strong promoter may…

Q: A series of exonuclease deletions were used to study the promoter of the rice hemA gene, giving the…

A: A promoter can be defined as a DNA sequence where proteins bind and initiate transcription of an…

Q: You construct a translational reporter gene fusion of your favourite gene. How can you assess…

A: Translational Gene Fusion is employed to live protein localization by quantifying promoter activity.…

Q: 61. Which pairs of restriction enzymes would allow the cloning of the HGH cDNA 3' (downstream) of…

A: Nucleases are enzymes that degrade nucleic acids. They can be classified intoRNase   : enzymes that…

Q: In E. coli, the gene bioD+ encodes an enzyme involved in biotin synthesis, and galK+ encodes an…

A: Transduction is a process of gene transfer in which DNA (deoxyribonucleic acid) from a source is…

Q: Parts A-C show the genotypes for partial diploid strains of the lac operon. Gene names have been…

A: (a) I-P+O+Z+Y:- The I- allele means the lac repressor is inactive, so the lac operon is…

Q: A rice MADS box transcription factor gene contains a number of sequences that are removed in the…

A: In eukaryotes including rice mRNA processing involves the removal of introns which are non-coding…

Q: Although a large number of mutagenic chemicals are known,none is known that induces mutations in…

A: Mutation is the process of abrupt changes in the nucleotide sequence or gene in the DNA. Mutation…

Q: When an EcoR1 fragment, which represents the coding region of a human gene X, is cloned into the…

A: Fusion proteins are created by combining two or more genes into a single gene. GST is a common…

Q: You are working with a laboratory strain of E.coli that contains a 10 base-pair deletion in the Lac…

A: The lac operator is the negative regulatory site in the lac operon of E.coli. Here the repressor…

Q: ou have isolated different mutants (reg1 and reg2) causing constitutive expression of the emu operon…

A: The operon's regulation can take place positively or negatively. If the negative operator is bound…

Q: You have isolated a mutant that you call Mutant Z. In a Mutant Z strain, the RNA polymerase can bind…

A: Here, the correct answer is option c) domain 2 of sigma and d) 3/4 linker of sigma.

Q: How to incorporate prokaryotic gene into eukaryotic cell to make an transgenic organism?In this case…

A: Transformation is a technique used to incorporate foreign DNA into a target organism. In this case,…

Q: Scientists who study amino acid biosynthesis pathwayswant to isolate auxotrophic bacteria. A…

A: Protein enrichment is a method by which the proteins of a specific type present in the sample are…

Q: An EMS mutagenesis screen identified several mutants in this gene. The figure shows the position of…

A: "As per the Q&A guidelines, we will provide the solution to the first question. Please repost…

Q: In E. coli, the gene bioD+ encodes an enzyme involved in biotin synthesis, and galK+ encodes an…

A: Gene transfer is the process of insertion of unrelated DNA into cells. There are three mechanisms of…

Q: In bacteria, genes that are often used together are controlled by a single promoter. Explain why…

A: Gene regulation refers to the numerous processes that regulate which genes are expressed and the…

Q: yeast cells, telomerase remains active and maintains telomeres of about 300 base pairs. Propose what…

A: The end areas of chromosomes are called telomeres , are repetitive sequence that protect gene from…

Q: A full-length eukaryotic gene is inserted into a bacterial chromosome.  The gene contains a complete…

A: Transcription in eukaryotes occurs in three consequent stages that start with the initiation step,…

Q: Strain P77 has a mutation in the lacO that prevents it from being bound by the lacI protein.…

A: Gene regulation at the level of transcription in bacteria is achieved by the operon model. Operon…

Q: You want to clone the gene Dik1 into a plasmid that will be used to generate a transgenic mouse. The…

A:

Q: If a wild-type (normal, NOTmutated) E. coli strain is grown in a medium: a. without lactose or…

A: The lac operon or the lactose operon is a mechanism that is involved in the metabolism and transport…

Q: A partial diploid in E. coli is created so that LacI is no longer expressed from the genome and is…

A: Partial diploid E.coli has both chromosomal DNA (i.e. genome) and plasmid DNA, but all the genes are…

Q: 9. Give the levels of Beta-galactosidase activity (high or low or zero) expected for the following…

A: A set of genes present in E.coli. that regulates the uptake of lactose from the surrounding…

Viral Replication

The virus is acellular and considered non-living outside the host cell. The replication of the virus occurs only inside the living cells. Only a few genes are present in the smallest virus, whereas many genes are present in the largest virus. These genes encode specific viral proteins.