Experiment 1 2 3 4 5 6 7 Components CTFIIE CTFIIH + CTFIIF CTFIIB + cPol II pPol II PTFIIB PTFIIE + PTFIIH + PTFIIF Transcription at S. pombe start site (30 bp downstream from TATA box) Transcription at S. cerevisiae start site (40-120 bp downstream from TATA box) Gel
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
The locations of the TATA box in two species of yeast, Saccharomyces pombe and Saccharomyces cerevisiae, differ dramatically. The TATA box of S. pombe is about 30
Q. Propose a mechanism that might have caused the transcription start site in S. pombe to be about 30 bp downstream of the TATA box, while the start site for transcription in S. cerevisiae is 40 to 120 bp downstream of the TATA box.
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