After performing analysis of whole genome sequencing from a candidate that grew on SC-H in the initial screen, you are unable to identify a causative mutation.  Which of the following options is a likely explanation for why this mutant passed the initial screen?  Select all that apply. A. The mutation is a truncation mutation and Illumina sequencing only provides information for missense mutations. B. One of the missense mutations you analyzed is actually causative, but the factor has not been previously linked to transcription. C. The causative mutation lies in a sequence coverage gap. D. The mutation is in a hypothetical protein and uncategorized genes cannot be sequenced by Illumina technology.

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A: cDNA  It is known as complementary DNA and made up of reverse transcription of mRNA.

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A: Gel electrophoresis is a laboratory technique for separating charged molecules such as DNA, RNA, and…

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A: Blot A shows a band for both the wild-type and IT gene, indicating that the ΔIT gene is still…

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Q: Compatible ends generated by restriction enzyme can be connected by ligase. Determine which of the…

A: Restriction enzymes are proteins that cut DNA at specific sequences. These sequences are usually…

Q: Your mutagenized sample showed 523 CFU after plating 100uL of media on the 10^3 dilution plate.  The…

A: CFU/ml = CFU/(Dilution × Volume plated)  CFU stands for colony forming units.

Q: A neutral mutation is, by definition, a mutation that does not result in the change of the encoded…

A: Neutral mutations are changes that occur in the DNA sequence which are neither beneficial or…

Q: What is restriction fragment length polymorphism and which method do we use to detect it?

A: Restrictions length polymorphism (RFLP) is a kind of polymorphism that outcomes from variety in the…

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Q: An EMS mutagenesis screen identified several mutants in this gene. The figure shows the position of…

A: "As per the Q&A guidelines, we will provide the solution to the first question. Please repost…

Q: The restriction digests of the cloned Drosophila gene can provide direct visible evidence of a…

A: We can use the technique of Southern blotting to detect and isolate the DNA fragment with the Gene…

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A:

Q: A possible forward primer to be designed is a primer that binds to the region of the OXA-M290 gene…

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A: Restriction enzymes are molecular scissors.

Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.