It was suspected that the cause of the infertility seen in a patient was due to undiagnosed non- classic late onset congenital adrenal hyperplasia.  In order to determine the presence of a specific mutation in the patient, PCR amplification of the CYP21 gene was undertaken on a DNA sample from patient. The amplified DNA was subsequently digested with a restriction enzyme (RE). A normal gene has four cut sites for the RE, however a gene with this specific mutation would show the loss of one of the cut sites.   After the DNA fragments have been separated by agarose gel electrophoresis, if the patient was heterozygous for the mutation, indicate how many fragments you would most likely expect to see on the gel.    Select ONE option: 2 3 4 5 6 7 8 9

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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It was suspected that the cause of the infertility seen in a patient was due to undiagnosed non- classic late onset congenital adrenal hyperplasia.  In order to determine the presence of a specific mutation in the patient, PCR amplification of the CYP21 gene was undertaken on a DNA sample from patient. The amplified DNA was subsequently digested with a restriction enzyme (RE). A normal gene has four cut sites for the RE, however a gene with this specific mutation would show the loss of one of the cut sites.

 

After the DNA fragments have been separated by agarose gel electrophoresis, if the patient was heterozygous for the mutation, indicate how many fragments you would most likely expect to see on the gel.   

Select ONE option:

2

3

4

5

6

7

8

9

 

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