Wnt4 is expressed in a variety of embryonic tissues. You decide to study this gene during mouse development Part 1) In the mouse embryo at gastrulations are Wnts concentrated at the anterior or posterior region? Part 2) You decide to conduct a Wnt4
Wnt4 is expressed in a variety of embryonic tissues. You decide to study this gene during mouse development Part 1) In the mouse embryo at gastrulations are Wnts concentrated at the anterior or posterior region? Part 2) You decide to conduct a Wnt4 immunoblot (Western blot) using tissues from mouse embryos. What key reagent do you need to carry out this method? Part 3) Your immunoblot shows that Wnt4 is expressed in the embryonic ovary. What also need to be expressed in the ovary for Wnt4 to have an effect there? Part 4) Outline in three dot points how Wnt4 can induce gene expression in neighbouring cells. Part 5) a. What method you would use to show that Wnt4 gene is required for ovary development in the mouse? b. In three dot point, outline how this method is carried out.
Step by step
Solved in 3 steps
