To investigate whether miR-31 regulates HDAC, the experiment shown in the figure below was carried out. CPWA Cells N.C + si-Dicer + + miR-31 + Dicer HDAC2 GAPDH Figure. Western blot analysis of CPWA cells were transfected with miR-31 mimics after transfection with Dicer siRNA or negative control siRNA (N.C). The protein levels of HDAC2 and Dicer were detected with their specific antibodies. Briefly describe the experiment, the results, AND the conclusions (including the relevance of including si-Dicer in the experiment). Hint: First, think about what is happening in first reaction (left) followed by the second and then the third.

To investigate whether miR-31 regulates HDAC, the experiment shown in the figure below was carried out. CPWA Cells N.C + si-Dicer + + miR-31 + Dicer HDAC2 GAPDH Figure. Western blot analysis of CPWA cells were transfected with miR-31 mimics after transfection with Dicer siRNA or negative control siRNA (N.C). The protein levels of HDAC2 and Dicer were detected with their specific antibodies. Briefly describe the experiment, the results, AND the conclusions (including the relevance of including si-Dicer in the experiment). Hint: First, think about what is happening in first reaction (left) followed by the second and then the third.

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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According to the research article: “Telomerase Modulates Wnt Signalling by Association with Target Gene Chromatin,” how did the authors determine that TERT, Brg1, and beta catenin were all part of the same protein complex on TCF sites in vivo? Describe the assay used to determine this.
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