The protein NK2 is a transcriptional regulator that functions both as a repressor and activator. To map the two domains more precisely a research group introduced deletion mutations within the coding sequences and studied the effect of a mutation on the NK2 regulatory function. A reporter construct (b-gal activity measurement) was used to monitor gene expression (row 15). Expression in the absence of NK2 was set at 100% (row 15). Repression is indicated by a significant reduction in expression (see row 14 for example) and activation is indicated by higher that 100% activity (e. g., row 11). The presence of the wild type NK2 led to near complete inhibition of expression (row 14). In the figure a deletion is shown as an open area and the residues deleted are indicated on the left (e.g., the row 13 mutant has a deletion of residues 1-97). - a) Which region(s) of the protein is required for repression function? - b) Is the C-terminal end required for repression? - c) What is the minimum region needed for repression? - d) Is the region labelled ‘HD’ sufficient for activation? - e) What is the minimum region needed for activation? (A region refers to an amino acid segment, e.g., aa 15-275)
The protein NK2 is a transcriptional regulator that functions both as a repressor and activator. To map the two domains more precisely a research group introduced deletion mutations within the coding sequences and studied the effect of a mutation on the NK2 regulatory function. A reporter construct (b-gal activity measurement) was used to monitor gene expression (row 15).
Expression in the absence of NK2 was set at 100% (row 15). Repression is indicated by a significant reduction in expression (see row 14 for example) and activation is indicated by higher that 100% activity (e. g., row 11). The presence of the wild type NK2 led to near complete inhibition of expression (row 14). In the figure a deletion is shown as an open area and the residues deleted are indicated on the left (e.g., the row 13 mutant has a deletion of residues 1-97).
- a) Which region(s) of the protein is required for repression function?
- b) Is the C-terminal end required for repression?
- c) What is the minimum region needed for repression?
- d) Is the region labelled ‘HD’ sufficient for activation?
- e) What is the minimum region needed for activation?
(A region refers to an amino acid segment, e.g., aa 15-275)
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