The protein NK2 is a transcriptional regulator that functions both as a repressor and activator. To map the two domains more precisely a research group introduced deletion mutations within the coding sequences and studied the effect of a mutation on the NK2 regulatory function. A reporter construct (b-gal activity measurement) was used to monitor gene expression (row 15). Expression in the absence of NK2 was set at 100% (row 15). Repression is indicated by a significant reduction in expression (see row 14 for example) and activation is indicated by higher that 100% activity (e. g., row 11). The presence of the wild type NK2 led to near complete inhibition of expression (row 14). In the figure a deletion is shown as an open area and the residues deleted are indicated on the left (e.g., the row 13 mutant has a deletion of residues 1-97). - a) Which region(s) of the protein is required for repression function? - b) Is the C-terminal end required for repression? - c) What is the minimum region needed for repression? - d) Is the region labelled ‘HD’ sufficient for activation? - e) What is the minimum region needed for activation? (A region refers to an amino acid segment, e.g., aa 15-275)
The protein NK2 is a transcriptional regulator that functions both as a repressor and activator. To map the two domains more precisely a research group introduced deletion mutations within the coding sequences and studied the effect of a mutation on the NK2 regulatory function. A reporter construct (b-gal activity measurement) was used to monitor gene expression (row 15).
Expression in the absence of NK2 was set at 100% (row 15). Repression is indicated by a significant reduction in expression (see row 14 for example) and activation is indicated by higher that 100% activity (e. g., row 11). The presence of the wild type NK2 led to near complete inhibition of expression (row 14). In the figure a deletion is shown as an open area and the residues deleted are indicated on the left (e.g., the row 13 mutant has a deletion of residues 1-97).
- a) Which region(s) of the protein is required for repression function?
- b) Is the C-terminal end required for repression?
- c) What is the minimum region needed for repression?
- d) Is the region labelled ‘HD’ sufficient for activation?
- e) What is the minimum region needed for activation?
(A region refers to an amino acid segment, e.g., aa 15-275)
![1 RD-Hom
-234567890123
11
12
13
14
15
A(167-277)
A(194-277)
A(97-138)
A(97-167)
A(97-194)
N595A
A(619-723)
HD
HA
I
I
A(1-392)
A(1-277) I
A(1-194) I
A(1-97) I
Wt NK-2
pUC41-75hsp40 Bgal
0
HD
100
150
200
Normalized % of B-gal activity
50
250
Figure 1. Effect of deletion in NK-2 on its transcription regulatory functions. The
rectangles identify the segments present. Sequences deleted are shown as open areas.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fb0d56cf4-ae0c-4387-a2fc-13a29f1b7e32%2F9e916e26-345d-48cb-aeb2-9ba08bab22e4%2Fnzxnqad_processed.png&w=3840&q=75)
![](/static/compass_v2/shared-icons/check-mark.png)
Trending now
This is a popular solution!
Step by step
Solved in 3 steps
![Blurred answer](/static/compass_v2/solution-images/blurred-answer.jpg)