The transcription factor Pax6 is required continually during the life of a mouse (or a human) for the development of the retina. Homozygous Pax6 knockout mice die soon after birth because Pax6 protein is also required in essential organs, such as the pancreas. a) In order to study the role of Pax6 in eye development a researcher wants to generate a mouse that expresses Pax6 everywhere except in its eyes. Describe how you could construct such a mouse by floxing the gene. Is it possible to achieve the same end with a transgene? (Hint: think about using cDNA and RNAI) b) Suppose you want to create a mouse similar to that in part (a), but one where the eye cells from Pax6 function has been removed and now express a gene that specifies a green fluorescent protein (GFP). Marking the cells in this way will allow the investigators to see the shapes of the Pax6- eye cells more easily than if they did not express GFP. Diagram a Pax6 gene construct that would enable the researcher to do this experiment by floxing Pax6.
The transcription factor Pax6 is required continually during the life of a mouse (or a human) for the development of the retina. Homozygous Pax6 knockout mice die soon after birth because Pax6 protein is also required in essential organs, such as the pancreas. a) In order to study the role of Pax6 in eye development a researcher wants to generate a mouse that expresses Pax6 everywhere except in its eyes. Describe how you could construct such a mouse by floxing the gene. Is it possible to achieve the same end with a transgene? (Hint: think about using cDNA and RNAI) b) Suppose you want to create a mouse similar to that in part (a), but one where the eye cells from Pax6 function has been removed and now express a gene that specifies a green fluorescent protein (GFP). Marking the cells in this way will allow the investigators to see the shapes of the Pax6- eye cells more easily than if they did not express GFP. Diagram a Pax6 gene construct that would enable the researcher to do this experiment by floxing Pax6.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:The transcription factor Pax6 is required continually during the life of a mouse (or a human)
for the development of the retina. Homozygous Pax6 knockout mice die soon after birth
because Pax6 protein is also required in essential organs, such as the pancreas.
a) In order to study the role of Pax6 in eye development a researcher wants to
generate a mouse that expresses Pax6 everywhere except in its eyes. Describe
how you could construct such a mouse by floxing the gene. Is it possible to
achieve the same end with a transgene? (Hint: think about using cDNA and
RNAI)
b) Suppose you want to create a mouse similar to that in part (a), but one where the
eye cells from Pax6 function has been removed and now express a gene that
specifies a green fluorescent protein (GFP). Marking the cells in this way will
allow the investigators to see the shapes of the Pax6- eye cells more easily than
if they did not express GFP. Diagram a Pax6 gene construct that would enable
the researcher to do this experiment by floxing Pax6.
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