Using a transgenic technique, propose an experiment to determine whether Cdx2 is sufficient for trophoblast development in the mouse embryo. Describe two results that you would expect to observe at the blastocyst stage if Cdx2 is indeed sufficient for trophoblast development. Be as specific as possible regarding the transgene that you propose for this experiment (including what gene's enhancer you would use in the transgene). Note: you do not need to explain the details of how a transgenic mouse is made. Describe the experiment in steps (Step 1: ..., Step 2: ... etc) and please keep your answer to under 150 words. tips: DONT talk about stop cassetes/memory cassetes, focus on transgenes Paper called "Cdx2 is required for correct cell fate specification and differentiation of trophectoderm in the mouse blastocyst" gave lots of results that you might see,, 6 diff ways that cdx2 is required for trophoblasts need specific gene enhancer (dont just say "expressed enhancer in genital ridge") gain of function experiment might be recommended
Using a transgenic technique, propose an experiment to determine whether Cdx2 is sufficient for trophoblast development in the mouse embryo. Describe two results that you would expect to observe at the blastocyst stage if Cdx2 is indeed sufficient for trophoblast development. Be as specific as possible regarding the transgene that you propose for this experiment (including what gene's enhancer you would use in the transgene). Note: you do not need to explain the details of how a transgenic mouse is made. Describe the experiment in steps (Step 1: ..., Step 2: ... etc) and please keep your answer to under 150 words.
tips:
-
DONT talk about stop cassetes/memory cassetes, focus on transgenes
-
Paper called "Cdx2 is required for correct cell fate specification and differentiation of trophectoderm in the mouse blastocyst" gave lots of results that you might see,, 6 diff ways that cdx2 is required for trophoblasts
-
need specific gene enhancer (dont just say "expressed enhancer in genital ridge")
- gain of function experiment might be recommended
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