Answer the following questions based on the information given below:
Act>CD2>Gal4 is a Flip-out construct that will express CD2 under the control of the ubiquitous Actin promoter in the absence of FLP. Expression of FLP will cause excision of the CD2 cassette so that GAL4 will be expressed instead. Suppose you identified a new gene X and generated UAS-X flies. In an experiment to examine the possible functions of X in eye development, you used flies with the following genotype:
HsFLP; Act>CD2>Gal4, UAS-GFP / +; UAS-X /+
After a brief heat shock to induce FLP expression, you went on to examine eye discs for cells that express gene X.
- How can you identify cells that have the expression of gene X? (1 point)
I would identify cells that have the expression of gene X through an Epistatic screen
- in the following image, you stained eye disc with antibodies against atonal (shown in Red), Elav (shown in blue), and GFP (shown in green). The yellow arrows point to the morphogenetic furrow (MF). The single-channel images of atonal and Elav are shown in (B) and (C). Please point to an area of the eye disc that has expression of X in Fig. A.
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