mouse embryo at gastrulation, are Wnts concentrated at the anterior or posterior region? ecide to conduct a Wnt4 immunoblot (Western blot), using tissues from mouse embryos. tey reagent do you need to carry out this method?
mouse embryo at gastrulation, are Wnts concentrated at the anterior or posterior region? ecide to conduct a Wnt4 immunoblot (Western blot), using tissues from mouse embryos. tey reagent do you need to carry out this method?
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:Wnt4 is expressed in a variety of embryonic tissues. You decide to study this gene during mouse development
Part 1
In the mouse embryo at gastrulation, are Wnts concentrated at the anterior or posterior region?
Part 2
You decide to conduct a Wnt4 immunoblot (Western blot), using tissues from mouse embryos.
What key reagent do you need to carry out this method?
Part 3
Your immunoblot shows that Wnt4 is expressed in the embryonic ovary. What also needs to be expressed in the ovary for Wnt4 to have an effect there?
Part 4
Outline in three dot points how Wnt4 can induce gene expression in neighbouring cells.
Part 5
a. What method you would use to show that Wnt4 gene is required for ovary development in the mouse?
b. In three dot points, outline how this method is carried out.
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