6) The figure that follows shows DNA fingerprint analysis of the genomic DNA from semen associated with a rape (***) and from mouth swabs (somatic cells) of individuals 1-4. This analysis involves the PCR-amplification of six SSR loci, each from a different (nonhomologous) chromosome. All PCR primers used are 20 nucleotides long; the primers for each locus have fluorescent tags in a locus-specific color. In the gel, some bands are thicker than others because relatively more of the corresponding PCR product was obtained. The figure has dots aligned on both sides that you can use to compare the sizes of the bands using the edge of a piece of a paper as a guide. Black B) C) D) Red eng Purpla Orange Graan ● .. · 20 W || I 11 | 11 T I T | || T 11 I || ● • . ● . 60 Black Red ani Purple Orange Green A) Sperm are haploid, but the semen sample shows two different-sized PCR products for certain loci. How is this possible? Is any bcus on the x chromosome? If so, idently it Is any locus on the Y chromosome? If so, identify it. Explain why these results nonetheless provide any information that none of the four individuals is the rapist. What pattern would you expect by analyzing mouth swab DNA from the rapist?

Human Anatomy & Physiology (11th Edition)
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Chapter1: The Human Body: An Orientation
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The figure that follows shows DNA fingerprint analysis of the genomic DNA from semen associated with a rape (***)
and from mouth swabs (somatic cells) of individuals 1-4. This analysis involves the PCR-amplification of six SSR
loci, each from a different (nonhomologous) chromosome. All PCR primers used are 20 nucleotides long; the
primers for each locus have fluorescent tags in a locus-specific color. In the gel, some bands are thicker than
others because relatively more of the corresponding PCR product was obtained. The figure has dots aligned on
both sides that you can use to compare the sizes of the bands using the edge of a piece of a paper as a guide.
2
34
Table
ente
Purpla
Develo
I
T
T
Black
Red
Blue
Purple
***
Drange
Green
A) Sperm are haploid, but the semen sample shows two different-sized PCR products for certain loci. How is this
possible?
B)
Is any bcus on the x chromosome? If so, identify it.
Is any bocus on the Y chromosome? If so, identify it.
D) Explain why these results nonetheless provide any information that none of the four individuals is the rapist. What
pattern would you expect by analyzing mouth swab DNA from the rapist?
Eitor Suggestions: Showing
-
V
Transcribed Image Text:eview J Search (Alt + Q) 6) View Help Editing ✓ Merge Cells Split Cells Distribute v Table Styles ✓ The figure that follows shows DNA fingerprint analysis of the genomic DNA from semen associated with a rape (***) and from mouth swabs (somatic cells) of individuals 1-4. This analysis involves the PCR-amplification of six SSR loci, each from a different (nonhomologous) chromosome. All PCR primers used are 20 nucleotides long; the primers for each locus have fluorescent tags in a locus-specific color. In the gel, some bands are thicker than others because relatively more of the corresponding PCR product was obtained. The figure has dots aligned on both sides that you can use to compare the sizes of the bands using the edge of a piece of a paper as a guide. 2 34 Table ente Purpla Develo I T T Black Red Blue Purple *** Drange Green A) Sperm are haploid, but the semen sample shows two different-sized PCR products for certain loci. How is this possible? B) Is any bcus on the x chromosome? If so, identify it. Is any bocus on the Y chromosome? If so, identify it. D) Explain why these results nonetheless provide any information that none of the four individuals is the rapist. What pattern would you expect by analyzing mouth swab DNA from the rapist? Eitor Suggestions: Showing - V
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