What is the role of ampicillin in these plates? (Hint: amp is considered a selectable marker but what does that mean?) Some of the agar plates have arabinose in them. What is the role of arabinose in these plates? Arabinose can be described as a transcriptional enhancer. How do you think it helps coli express GFP? Relate it back to the basic way operons can be turned on/off.
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
- Some of the agar plates have ampicillin in them. What is the role of ampicillin in these plates? (Hint: amp is considered a selectable marker but what does that mean?) Some of the agar plates have arabinose in them. What is the role of arabinose in these plates?
- Arabinose can be described as a transcriptional enhancer. How do you think it helps coli express GFP? Relate it back to the basic way operons can be turned on/off.
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