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A. Add mutagen to culture of Saccharomyces cerevisiae B. Add mutagen to plate C. Incubate to allow colonies to grow D. Plate Saccharomyces cerevisiae on solid media containing Leu E. Plate Saccharomyces cerevisiae on solid media lacking Leu Replica plate onto solid media lacking Leu F. G. H. I. Replica plate onto solid media containing Leu Identify colonies that did not grow on the replica plate Identify colonies that grew on the replica plate

A. Add mutagen to culture of Saccharomyces cerevisiae B. Add mutagen to plate C. Incubate to allow colonies to grow D. Plate Saccharomyces cerevisiae on solid media containing Leu E. Plate Saccharomyces cerevisiae on solid media lacking Leu Replica plate onto solid media lacking Leu F. G. H. I. Replica plate onto solid media containing Leu Identify colonies that did not grow on the replica plate Identify colonies that grew on the replica plate

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Order the following experimental steps to identify auxotrophic mutants in Saccharomyces Cerevisiae that cannot sythesize their own Leucine. an option can be selected more than once. 

step 1: 

Step 2: 

Step 3: 

Step 4: 

Step 5: 

Step 6: 

A. Add mutagen to culture of Saccharomyces cerevisiae B. Add mutagen to plate C. Incubate to allow colonies to grow D. Plate Saccharomyces cerevisiae on solid media containing Leu E. Plate Saccharomyces cerevisiae on solid media lacking Leu Replica plate onto solid media lacking Leu F. G. H. I. Replica plate onto solid media containing Leu Identify colonies that did not grow on the replica plate Identify colonies that grew on the replica plate
Transcribed Image Text:A. Add mutagen to culture of Saccharomyces cerevisiae B. Add mutagen to plate C. Incubate to allow colonies to grow D. Plate Saccharomyces cerevisiae on solid media containing Leu E. Plate Saccharomyces cerevisiae on solid media lacking Leu Replica plate onto solid media lacking Leu F. G. H. I. Replica plate onto solid media containing Leu Identify colonies that did not grow on the replica plate Identify colonies that grew on the replica plate
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