30- 20- hisE phe4 10 0+ 10 20 30 40 50 Duration of conjugation (minutes) % of Freciplent cells that have recel ved the gene during corjuga tion
Genetic Recombination
Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.
Microbial Genetics
Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.
An Hfr strain that is hisE+ and pheA+ was mixed with a strain that
is hisE− and pheA−. The conjugation was interrupted and the percentage
of recombinants for each gene was determined by streaking
on a medium that lacked either histidine or phenylalanine. The
following results were obtained:
Determine the map distance (in minutes) between these two
genes.
Transfer of genes in prokaryotes occurs through horizontal transfer mechanisms such as transduction, transformation, and conjugation. Horizontal transfer of bacterial plasmid containing genetic material to similar or different bacterial species leads to genetic recombination.
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