Each of the three different Hfr strains in the table below (A, B and C) arose independentlyand possess the same markers. Each of the three Hfr strains was separately mixed with thesame F strain carrying the alternative markers. Conjugation was interrupted at various timesafter mixing over a period of 95 minutes. The bacteria were then plated on the appropriatemedia and recipients scored for various Hfr markers. The times (in minutes after mixing) atwhich recombinants carrying particular donor markers first appear, and therefore the times atwhich these first enter the F- cell and then integrate into the recipient genome, are shownbelow:DonormarkersTime of appearance in minutesStrain AStrain BStrain Chis+6.568.529.5gal+30.046.052.0pro+34.541.556.5met+58.020.076.0mtl+67.011.085.0xyl+68.59.586.5mal+73.05.03.0ser+81.586.511.5tyr+88.078.519.5Draw the genome of each strain showing the location of the markers, the location of F, andthe direction of transfer during conjugation.

A genome is the complete set of genetic information in an organism. It provides all of the…

Answered: Each of the three different Hfr strains…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

See similar textbooks

Similar questions

If a student uses a monoclonal antibody to detect a protein using a direct Western-blotting protocol after running an SDS PAGE, the student can detect a 50kd protein. However, if the student tries to use this antibody to immunoprecipitate the 50 kd protein and then Western -blot the immunoprecipitated samples, the student found that there is no band at all at 50kd region in the Western blot. How do you explain these results based on what you know about protein structure and antigen-antibody recognition? (Hint: Western blot analysis explores an SDS-containing buffer, whereas the immunoprecipitation buffer uses a NP40-containing buffer to maintain proteins in their native form).
An individual is heterozygous for an allele. Allele A has a SNP that results in disruption of an Not1 restriction enzyme site. Allele B has a functional Not1 restriction enzyme site. PCR is performed on the region of the gene containing the SNP to produce a 255bp PCR product. Restriction digestion was subsequently performed using EcoR1 restriction enzyme and the fragments were electrophoresed on an agarose gel. (EcoR1 cuts allele B at a site 115bp from the end of the sequence.) What are the sizes of the subsequent DNA fragments? 1) 115bp + 140bp 2) 205bp + 255bp 3) 140bp 4) 205bp + 255bp + 115bp 5) 140bp + 205bp + 255bp 6) 255bp + 115bp + 140bp 7) 115bp 8) 255bp 9) 115bp + 255bp 10) 255bp + 115bp + 140bp + 205bp 11) 140bp + 205bp 12) 205bp 13) 205bp + 115bp 14) 140bp + 255bp
You have four strains, and you test for growth on the following medias: Rich, Minimal, Minimal + Lysine, Minimal + Histidine, and Minimal +Lysine and Histidine. The table below shows your results. Strain Rich 1 2 3 4 Minimal Min + Lysine Strain 2 Growth No Growth Growth Growth No Growth No Growth Strain 4 Growth Growth Strain 1 Lysine auxotroph only Growth Histidine auxotroph only Strain 3 is not an auxotroph, but a pro Min + Histidine No Growth Is an auxotroph, but not for ly ✓ No Growth Growth Determine whether each strain is auxotroph or a prototroph, and if an auxotroph what sort of auxotroph? Min + Lysine and Histidine Growth No Growth No Growth No Growth No Growth Growth Growth Growth
All of the following pieces of information are expected to be obtained directly from Western Blot analysis, except: An estimation of the fusion protein’s size Relative quantity of the fusion protein compared to other samples The amino acid sequence of the fusion protein If the fusion protein is present in the sample
i need help finding the answer in the article How does the amount of antibiotics used at the farms tested compare with other farms around the world? What about the variety?
Explain the process of how X-gal screening works with pUC19, you may build a model with boxes and arrows. 2. You are utilizing BamHI (GGATCC) restriction site and HindIII (AAGCTT) restriction site. Within pUC19, BamHI is at position 263, while HindIII is at position 233. (Hint: position is like coordinate on a map). If you manage to insert GTF2H5 in pUC19 vector, what are the sizes of fragments if you digest the pUC19-GTF2H5 (this is after insertion) with the following restriction enzyme combination after gel electrophoresis: BamHI and HindIII There is an NdeI site right in the middle of the GTF2H5 that has been inserted in pUC19, what would be the fragment sizes, if you digest with BamHI and NdeI. HindIII and NdeI BamHI, HindIII, and NdeI. 3. Design an experiment to confirm the presence of insert GTF2H5 in pUC19 vector using the following method (besides restriction analysis above), assuming that you know the sequence of GTF2H5: Southern Blot Polymerase Chain Reaction

Recommended textbooks for you

Human Anatomy & Physiology (11th Edition)

Biology

ISBN:

9780134580999

Author:

Elaine N. Marieb, Katja N. Hoehn

Publisher:

PEARSON

Biology 2e

Biology

ISBN:

9781947172517

Author:

Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:

OpenStax

Anatomy & Physiology

Biology

ISBN:

9781259398629

Author:

McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter

Publisher:

Mcgraw Hill Education,

Human Anatomy & Physiology (11th Edition)

Biology

ISBN:

9780134580999

Author:

Elaine N. Marieb, Katja N. Hoehn

Publisher:

PEARSON

Biology 2e

Biology

ISBN:

9781947172517

Author:

Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:

OpenStax

Anatomy & Physiology

Biology

ISBN:

9781259398629

Author:

McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter

Publisher:

Mcgraw Hill Education,

Molecular Biology of the Cell (Sixth Edition)

Biology

ISBN:

9780815344322

Author:

Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter

Publisher:

W. W. Norton & Company

Laboratory Manual For Human Anatomy & Physiology

Biology

ISBN:

9781260159363

Author:

Martin, Terry R., Prentice-craver, Cynthia

Publisher:

McGraw-Hill Publishing Co.

Inquiry Into Life (16th Edition)

Biology

ISBN:

9781260231700

Author:

Sylvia S. Mader, Michael Windelspecht

Publisher:

McGraw Hill Education

SEE MORE TEXTBOOKS