Genes encoding toxins are often located on plasmids.A recent outbreak has just occurred in which a bacterium that is usually nonpathogenic is producing atoxin. Plasmid DNA can be isolated from this newlypathogenic bacterial strain and separated from thechromosomal DNA. To establish whether the plasmidDNA contains a gene encoding the toxin, you coulddetermine the sequence of the entire plasmid andsearch for a sequence that looks like other toxin genespreviously identified. An easier way exists to determine whether the plasmid DNA carries the gene(s)for the toxin; this strategy does not involve DNAsequence analysis. Describe this easier method.
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Genes encoding toxins are often located on plasmids.
A recent outbreak has just occurred in which a bacterium that is usually nonpathogenic is producing a
toxin. Plasmid DNA can be isolated from this newly
pathogenic bacterial strain and separated from the
chromosomal DNA. To establish whether the plasmid
DNA contains a gene encoding the toxin, you could
determine the sequence of the entire plasmid and
search for a sequence that looks like other toxin genes
previously identified. An easier way exists to determine whether the plasmid DNA carries the gene(s)
for the toxin; this strategy does not involve DNA
sequence analysis. Describe this easier method.
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
