Acridine orange is a chemical that inhibits the replication ofF- factor DNA but does not affect the replication of chromosomalDNA, even if the chromosomal DNA contains an Hfr. Let’s supposethat you have an E. coli strain that is unable to metabolizelactose and has an F factor that carries a streptomycin-resistantgene. You also have an F− strain of E. coli that is sensitive to streptomycinand has the genes that allow the bacterium to metabolize lactose. This second strain can grow on a lactose-containing medium.How would you generate an Hfr strain that is resistant tostreptomycin and can metabolize lactose? (Hint: F factors occasionally integrate into the chromosome to become Hfr strains, and occasionally Hfr strains excise their DNA from the chromosome to become F+ strains that carry an F′ factor.)
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Acridine orange is a chemical that inhibits the replication of
F- factor DNA but does not affect the replication of chromosomal
DNA, even if the chromosomal DNA contains an Hfr. Let’s suppose
that you have an E. coli strain that is unable to
lactose and has an F factor that carries a streptomycin-resistant
gene. You also have an F− strain of E. coli that is sensitive to streptomycin
and has the genes that allow the bacterium to metabolize lactose. This second strain can grow on a lactose-containing medium.
How would you generate an Hfr strain that is resistant to
streptomycin and can metabolize lactose?
(Hint: F factors occasionally integrate into the chromosome to become Hfr strains, and occasionally Hfr strains excise their DNA from the chromosome to become F+ strains that carry an F′ factor.)
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