Norflaxacin is a cidal broad spectrum antibiotic that functions by inhibiting DNA gyrase in gram +/- cells. (DNA gyrase reverses supercoiling which occurs when DNA is unwound during replication). Why does Norflaxacin harm infecting bacteria but not human cells?

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A: In Bacteria Conjugation is dependent upon thetra genes found in 'conjugative' plasmids, which, among…

Q: Could you explain how you would know if you successfully induced transformation? Thank you Did you…

A: Transformation experiments are very crucial for understanding fundamental biological processes.…

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Q: How is the mechanism of excision repair similar to that of mismatch repair?

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Q: Plasmid DNA Extraction by Alkaline Lysis Why is Glacial acetic acid added to solution? What is the…

A: Plasmid  The small usually circular extra chromosomal DNA that contains extra gene for protection of…

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A: Introduction The non-sexual flow of genetic information between genomes is known as horizontal gene…

Q: In a cotransformation experiment DNA was isolated from a donor strain that was proA+ and strC+ and…

A: DNA (Deoxyribo-Nucleic acid) is the double stranded genetic material in all prokaryotic and…

Q: General recombination occurs in bacteria where it is involved in several types of intermicrobial DNA…

A: Genetic refers to relating to the genetic material. The genetic material of a cell is the DNA…

Q: Draw a diagram showing what pGEM will look like after it has been digested with BamHI.

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Q: How can you tell if you have had a successful induced transformation using: "C" = control, "T"…

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Q: Based on the following wild type DNA sequence, indicate if each of the mutations should be…

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A: Escherichia coli, commonly abbreviated as E. coli, is a gram-negative, rod-shaped bacterium that is…

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Q: In a cotransformation experiment (see question 4 of More GeneticTIPS), DNA was isolated from a donor…

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A: Introns are the non active genes present in the DNA.

Q: Analogs of nucleotides are often used in studies of DNA.  An analog is a modification of one of the…

A: Nucleotide analogs, are alterations of typical bases that can be joined into DNA and afterward…

Q: How many fragments and how big each fragment should be after digesting the plasmid with BamHI? How…

A: Digestion of the pUC19 plasmid using the restriction enzymes BamHI and PvuI.1.BamHI Digestion:BamHI…

Q: Please provide explanation for each step

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A: There are many agents which result in double-stranded breaks in DNA. These involves oxidizing…

Q: Which of the following statement is true? * Restriction enzyme binds to non-cognate DNA better than…

A: Restriction enzymes also known as restriction endonucleases are enzymes that recognize and cleave…

Q: plasmid DNA replication

A: C option is correct. In Hfr cell F factor is integrated into the chromosomal bacteria. During…

Q: Consider E, coli cells(i) with no vector,(ii) transformed with PBR322, or (iii) transformed with a…

A: pBR322 is a plasmid vector. It is double standard DNA molecule. It contains- Origin of replication…

Q: 5- Which statement is not true for agarose gel electrophoresis? a) DNA fragments are separated based…

A: The technique which is used for the separation of DNA fragments is called agarose gel…

Q: You are analyzing the intracellular DNA intermediates formed during the replication of the…

A: The provided experimental results involve the analysis of DNA intermediates in wild-type and mutant…

Q: Extrachromosomal DNA is critical to the antibiotic resistance found in microorganisms, how do these…

A: Extrachromosomal DNA means a DNA which is present independently of the main DNA or chromosome.…

Q: You have two DNA samples. Only one was expected to have plasmid DNA that was isolated from it. Both…

A: Since only one out of two samples has plasmid DNA. The sample with DNA of other forms can be…

Viral Replication

The virus is acellular and considered non-living outside the host cell. The replication of the virus occurs only inside the living cells. Only a few genes are present in the smallest virus, whereas many genes are present in the largest virus. These genes encode specific viral proteins.