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(2071) AhdI (2005) BsaI (2002) BpmI (1986) BsrFI (1924) NmeAIII .2000 (2655) BseYI PspFI (2659) pBR322ori-F (118 .. 137) (2550) AlwNI (1673) TsoI 2500 AmpR ori PUC19 2686 bp AflIII- PciI (273) lac p CAP binding si 500 L4440 (371.. 388) BspQI - SapI (390) M13/PUC Reverse (584 .. 606) M13 Reverse (603.. 619) HindIII(632) BfUAI- BspMI (637) SphI (642) PstI SbfI (648) SalI (650) BamHI (662) GGATCC 1 site 1000 lacza CCTAGG AccI (651) HincII(652) XbaI (656) BamHI (662) AvaI BsoBITspMI-XmaI (667) meT1101 (668) maI (669) cc651 (671) KpnI (675) Eco53kI (679) BanII- SacI (681) ApoI - EcoRI (683) M13 Forward (689.. 706) M13/PUC Forward (698 .. 720) KasI (844) NarI (845) M13 rev MCS AmpR promoter sig... 1500 SfoI (846) PluTI(848) NdeI (897) BstAPI (901) PRS-marker (914.. 933) PfoI (1032) pGEX 3' (1033.. 1055) Eco01091 (1091) PBRforEco (1093.. 1111) ZraI (1150) AatII (1152) (1590) ScaI (1502 1521) Amp-R (1471) XmnI SspI (1266) Enzyme PvuI PvuII Cut Site (bp) 806, 1702 453, 775 Total Cuts 2 2

(2071) AhdI (2005) BsaI (2002) BpmI (1986) BsrFI (1924) NmeAIII .2000 (2655) BseYI PspFI (2659) pBR322ori-F (118 .. 137) (2550) AlwNI (1673) TsoI 2500 AmpR ori PUC19 2686 bp AflIII- PciI (273) lac p CAP binding si 500 L4440 (371.. 388) BspQI - SapI (390) M13/PUC Reverse (584 .. 606) M13 Reverse (603.. 619) HindIII(632) BfUAI- BspMI (637) SphI (642) PstI SbfI (648) SalI (650) BamHI (662) GGATCC 1 site 1000 lacza CCTAGG AccI (651) HincII(652) XbaI (656) BamHI (662) AvaI BsoBITspMI-XmaI (667) meT1101 (668) maI (669) cc651 (671) KpnI (675) Eco53kI (679) BanII- SacI (681) ApoI - EcoRI (683) M13 Forward (689.. 706) M13/PUC Forward (698 .. 720) KasI (844) NarI (845) M13 rev MCS AmpR promoter sig... 1500 SfoI (846) PluTI(848) NdeI (897) BstAPI (901) PRS-marker (914.. 933) PfoI (1032) pGEX 3' (1033.. 1055) Eco01091 (1091) PBRforEco (1093.. 1111) ZraI (1150) AatII (1152) (1590) ScaI (1502 1521) Amp-R (1471) XmnI SspI (1266) Enzyme PvuI PvuII Cut Site (bp) 806, 1702 453, 775 Total Cuts 2 2

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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  1. How many fragments and how big each fragment should be after digesting the plasmid with

    BamHI?

  2. How many fragments and how big each fragment should be after digesting the plasmid with

    PvuI?

  3. How many fragments and how big each fragment should be after digesting with both enzymes?

    Show your calculation/explanation.

(2071) AhdI (2005) BsaI (2002) BpmI (1986) BsrFI (1924) NmeAIII .2000 (2655) BseYI PspFI (2659) pBR322ori-F (118 .. 137) (2550) AlwNI (1673) TsoI 2500 AmpR ori PUC19 2686 bp AflIII- PciI (273) lac p CAP binding si 500 L4440 (371.. 388) BspQI - SapI (390) M13/PUC Reverse (584 .. 606) M13 Reverse (603.. 619) HindIII(632) BfUAI- BspMI (637) SphI (642) PstI SbfI (648) SalI (650) BamHI (662) GGATCC 1 site 1000 lacza CCTAGG AccI (651) HincII(652) XbaI (656) BamHI (662) AvaI BsoBITspMI-XmaI (667) meT1101 (668) maI (669) cc651 (671) KpnI (675) Eco53kI (679) BanII- SacI (681) ApoI - EcoRI (683) M13 Forward (689.. 706) M13/PUC Forward (698 .. 720) KasI (844) NarI (845) M13 rev MCS AmpR promoter sig... 1500 SfoI (846) PluTI(848) NdeI (897) BstAPI (901) PRS-marker (914.. 933) PfoI (1032) pGEX 3' (1033.. 1055) Eco01091 (1091) PBRforEco (1093.. 1111) ZraI (1150) AatII (1152) (1590) ScaI (1502 1521) Amp-R (1471) XmnI SspI (1266)
Transcribed Image Text:(2071) AhdI (2005) BsaI (2002) BpmI (1986) BsrFI (1924) NmeAIII .2000 (2655) BseYI PspFI (2659) pBR322ori-F (118 .. 137) (2550) AlwNI (1673) TsoI 2500 AmpR ori PUC19 2686 bp AflIII- PciI (273) lac p CAP binding si 500 L4440 (371.. 388) BspQI - SapI (390) M13/PUC Reverse (584 .. 606) M13 Reverse (603.. 619) HindIII(632) BfUAI- BspMI (637) SphI (642) PstI SbfI (648) SalI (650) BamHI (662) GGATCC 1 site 1000 lacza CCTAGG AccI (651) HincII(652) XbaI (656) BamHI (662) AvaI BsoBITspMI-XmaI (667) meT1101 (668) maI (669) cc651 (671) KpnI (675) Eco53kI (679) BanII- SacI (681) ApoI - EcoRI (683) M13 Forward (689.. 706) M13/PUC Forward (698 .. 720) KasI (844) NarI (845) M13 rev MCS AmpR promoter sig... 1500 SfoI (846) PluTI(848) NdeI (897) BstAPI (901) PRS-marker (914.. 933) PfoI (1032) pGEX 3' (1033.. 1055) Eco01091 (1091) PBRforEco (1093.. 1111) ZraI (1150) AatII (1152) (1590) ScaI (1502 1521) Amp-R (1471) XmnI SspI (1266)
Enzyme PvuI PvuII Cut Site (bp) 806, 1702 453, 775 Total Cuts 2 2
Transcribed Image Text:Enzyme PvuI PvuII Cut Site (bp) 806, 1702 453, 775 Total Cuts 2 2
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