Compare and contrast the growth (# of colonies or lawn or no growth) on each of these pairs of plates. What do you (or should you) get one each, and, most importantly, what does each pair tell you (what do you learn by these comparisons?) LB AMP no plasmid vs. LB no plasmid LB AMP + plasmid vs. LB AMP no plasmid LB no plasmid vs. LB AMP + plasmid
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Compare and contrast the growth (# of colonies or lawn or no growth) on each of these pairs of plates. What do you (or should you) get one each, and, most importantly, what does each pair tell you (what do you learn by these comparisons?)
- LB AMP no plasmid vs. LB no plasmid
- LB AMP + plasmid vs. LB AMP no plasmid
- LB no plasmid vs. LB AMP + plasmid
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