You clone a fragment of DNA into the middle of the lacZ sequence of a plasmid, introduce the plasmid into bacteria, and then plate the bacterial colonies with ampicillin and X-gal. If you select blue colonies rather than white colonies, you will isolate bacterial colonies that O contain only the cloned piece of DNA. O contain the plasmid plus a cloned piece of DNA. O are not ampicillin-resistant. O contain only the plasmid. X

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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You clone a fragment of DNA into the middle of the lacZ sequence of a plasmid, introduce the plasmid into bacteria, and
then plate the bacterial colonies with ampicillin and X-gal. If you select blue colonies rather than white colonies, you will
isolate bacterial colonies that
O contain only the cloned piece of DNA.
O contain the plasmid plus a cloned piece of DNA.
O are not ampicillin-resistant.
O contain only the plasmid.
X
Transcribed Image Text:You clone a fragment of DNA into the middle of the lacZ sequence of a plasmid, introduce the plasmid into bacteria, and then plate the bacterial colonies with ampicillin and X-gal. If you select blue colonies rather than white colonies, you will isolate bacterial colonies that O contain only the cloned piece of DNA. O contain the plasmid plus a cloned piece of DNA. O are not ampicillin-resistant. O contain only the plasmid. X
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