You have two DNA samples. Only one was expected to have plasmid DNA that was isolated from it. Both DNA samples were exposed to two different treatment conditions: untreated, or treated with DNase I. How should the DNase I treatment change how DNA appears on the gel?
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
You have two DNA samples. Only one was expected to have plasmid DNA that was isolated from it. Both DNA samples were exposed to two different treatment conditions: untreated, or treated with DNase I.
How should the DNase I treatment change how DNA appears on the gel?
Since only one out of two samples has plasmid DNA. The sample with DNA of other forms can be single-stranded or double-stranded, readily cleaved by DNase I. Whereas the one with plasmid DNA, the highly supercoiled, is not easily affected by DNase I.
In a few reports, there are instances of plasmid DNA acted by DNase I, but it depends on the ionic strength of the buffer and treatment duration.
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