You are doing a cloning and transformation experiment. You insert your gene of interest into a plasmid that contains ampicillin resistance and transform it into your model organism.What plate would you expect to have no colonies, a negative control? p. A plate that contained ampicillin and the original uncut plasmid. q. A plate that contained ampicillin and the cut plasmid. r. A plate that contained ampicillin and the plasmid with the desired gene inserted A plate that contained no ampicillin and the original uncut plasmid. A plate that contained no ampicillin and the plasmid with the desired gene inserted

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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**Cloning and Transformation Experiment Overview**

In this experiment, you insert your gene of interest into a plasmid containing ampicillin resistance, transforming it into your model organism. To determine which plate serves as a negative control (one with no colonies expected), consider the following plates:

- **p.** A plate with ampicillin and the original uncut plasmid.
- **q.** A plate with ampicillin and the cut plasmid.
- **r.** A plate with ampicillin and the plasmid with the desired gene inserted.
- **s.** A plate with no ampicillin and the original uncut plasmid.
- **t.** A plate with no ampicillin and the plasmid with the desired gene inserted.

The highlighted options (s and t) denote plates lacking ampicillin. These plates would typically not serve as the expected choice for negative controls, as they allow for the growth of bacteria regardless of transformation efficiency.
Transcribed Image Text:**Cloning and Transformation Experiment Overview** In this experiment, you insert your gene of interest into a plasmid containing ampicillin resistance, transforming it into your model organism. To determine which plate serves as a negative control (one with no colonies expected), consider the following plates: - **p.** A plate with ampicillin and the original uncut plasmid. - **q.** A plate with ampicillin and the cut plasmid. - **r.** A plate with ampicillin and the plasmid with the desired gene inserted. - **s.** A plate with no ampicillin and the original uncut plasmid. - **t.** A plate with no ampicillin and the plasmid with the desired gene inserted. The highlighted options (s and t) denote plates lacking ampicillin. These plates would typically not serve as the expected choice for negative controls, as they allow for the growth of bacteria regardless of transformation efficiency.
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