he enzymes required to breathe fire (BFi2) is expressed in throat tissues of dragons from the Known World (comprising Westeros, Essos, Sothyros and Ulthos) at various times during their life cycles. A test very similar to DNase I hypersensitivity is conducted on embryonic (E) and adult (A) throat tissue samples of Valeriyan, Ice and Sea dragons. In the analysis, a radioactive label is attached to one end of each chromosome fragment, and the samples from each tissue are exposed to DNase I to determine if regions of BFi2 are DNase I hypersensitive. The content from each sample is then separated by gel electrophoresis and the results are shown below.
31. The enzymes required to breathe fire (BFi2) is expressed in throat tissues of dragons from the Known World (comprising Westeros, Essos, Sothyros and Ulthos) at various times during their life cycles. A test very similar to DNase I hypersensitivity is conducted on embryonic (E) and adult (A) throat tissue samples of Valeriyan, Ice and Sea dragons. In the analysis, a radioactive label is attached to one end of each chromosome fragment, and the samples from each tissue are exposed to DNase I to determine if regions of BFi2 are DNase I hypersensitive. The content from each sample is then separated by gel electrophoresis and the results are shown below.
Based on the gel results, in which tissue(s) and at what times during development do the results indicate the expression of BFi2 is most likely taking place?
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