(1) Why the ratio of PE/GFP fluorescence is measured in the FACS experiment, instead of measuring only total PE fluorescence? (2) Explain the effect that each mutation causes in the function of MC4R, and how they can be linked to disease.
(1) Why the ratio of PE/GFP fluorescence is measured in the FACS experiment, instead of measuring only total PE fluorescence? (2) Explain the effect that each mutation causes in the function of MC4R, and how they can be linked to disease.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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(1) Why the ratio of PE/GFP fluorescence is measured in the FACS experiment, instead of measuring only total PE fluorescence?
(2) Explain the effect that each mutation causes in the function of MC4R, and how they can be linked to disease.
Transcribed Image Text:WT
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95
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Ratio PE/GFP (%WT)
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38
82
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100
33
69
99
![Plot showing the different levels of cAMP produced upon stimulation of cells transfected with the different mutants and WT
pmol CAMP/106 cells
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1102T
WT
N240S
A P78L
:
le-10 1e-09 1e-08
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TTT
1e-07
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Transcribed Image Text:Plot showing the different levels of cAMP produced upon stimulation of cells transfected with the different mutants and WT
pmol CAMP/106 cells
700
600
500
400
300
200
100
L
0+
le-12
L
www
le-11
●
■
1102T
WT
N240S
A P78L
:
le-10 1e-09 1e-08
[NDP-MSH], M
TTT
1e-07
T
L
1e-06
700
600
500
400
300
200
100
0
1e-05
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