1. 3 4 64 7 10 250 250 150 150 100 100 75 75 Heavy Chain 50 50 37 37 Light Chain 25 25 Std 5.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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This is an SDS-PAGE of an antibody purification sample with IgG seperated from a Bovine Calf serum. Would you be able to describe the bands that are appearing and why it appeared on the gel this way? As well, a western blot was done after the SDS-PAGE and the bands that appeared were at 50 kDa and 150 kDa for the IgG and two elution lanes. May you please explain?

8
9
10
250-
250
150
-150
100
100
75
-75
Heavy Chain
50
37
Light Chain
25
-25
Std
Protein Standard
IgG
Elution
Unbound
Wash
Bovine Calf Serum
Elution
Unbound
Wash
6X LD
Transcribed Image Text:8 9 10 250- 250 150 -150 100 100 75 -75 Heavy Chain 50 37 Light Chain 25 -25 Std Protein Standard IgG Elution Unbound Wash Bovine Calf Serum Elution Unbound Wash 6X LD
Expert Solution
Step 1

SDS-PAGE is a technique for separation of proteins based on size. Antibody purification is done by allowing the anitobody to bind to an antigen followed by washing of unbound antibodies. Then the bound antibodies are eluted.

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