Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
Related questions
Question
This is an SDS-PAGE of an antibody purification sample with IgG seperated from a Bovine Calf serum. Would you be able to describe the bands that are appearing and why it appeared on the gel this way? As well, a western blot was done after the SDS-PAGE and the bands that appeared were at 50 kDa and 150 kDa for the IgG and two elution lanes. May you please explain?

Transcribed Image Text:8
9
10
250-
250
150
-150
100
100
75
-75
Heavy Chain
50
37
Light Chain
25
-25
Std
Protein Standard
IgG
Elution
Unbound
Wash
Bovine Calf Serum
Elution
Unbound
Wash
6X LD
Expert Solution
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Step 1
SDS-PAGE is a technique for separation of proteins based on size. Antibody purification is done by allowing the anitobody to bind to an antigen followed by washing of unbound antibodies. Then the bound antibodies are eluted.
Step by step
Solved in 2 steps
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