Question 14 A ten-fold increase in the concentration of sodium ions increases the Tm temperature of DNA by 16.66 °C. What is the mechanism of this process? a. Sodium ions dissociate double-stranded DNA by breaking hydrogen bonds between nitrogen bases. b. The presence of sodium ions increases the stability of double-stranded DNA. c. Sodium ions neutralize negative charges on single-stranded DNA. This increases the re-association reaction rate, thus increasing the Tm of DNA. d. Sodium ions prevent the re-association of single-stranded DNA molecules by forming strong hydrogen bonds with nitrogen bases, decreasing the rate of the re-annealing reaction.

Human Anatomy & Physiology (11th Edition)
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ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Question 14 A ten-fold increase in the concentration of sodium ions increases the Tm
temperature of DNA by 16.66 °C. What is the mechanism of this process?
a. Sodium ions dissociate double-stranded DNA by breaking hydrogen
bonds between nitrogen bases.
b.
The presence of sodium ions increases the stability of double-stranded
DNA.
c.
Sodium ions neutralize negative charges on single-stranded DNA. This
increases the re-association reaction rate, thus increasing the Tm of
DNA.
d. Sodium ions prevent the re-association of single-stranded DNA
molecules by forming strong hydrogen bonds with nitrogen bases,
decreasing the rate of the re-annealing reaction.
Transcribed Image Text:Question 14 A ten-fold increase in the concentration of sodium ions increases the Tm temperature of DNA by 16.66 °C. What is the mechanism of this process? a. Sodium ions dissociate double-stranded DNA by breaking hydrogen bonds between nitrogen bases. b. The presence of sodium ions increases the stability of double-stranded DNA. c. Sodium ions neutralize negative charges on single-stranded DNA. This increases the re-association reaction rate, thus increasing the Tm of DNA. d. Sodium ions prevent the re-association of single-stranded DNA molecules by forming strong hydrogen bonds with nitrogen bases, decreasing the rate of the re-annealing reaction.
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